Please I want to block ChREBP Ab (NB400-135) using ChREBP peptide I need help to no the procedure and how much I need to add from the peptide to the ChREBP Ab

Name: CHREBP Antibody Blocking Peptide
Brand: Novus Biologicals
SKU: NB400-135PEP
Rating: 4 (1 reviews)

Here is a protocol for a blocking peptide control for a western blot: Materials and Reagents Blocking buffer (usually TBST plus either 5% non-fat dry milk or 3% BSA for Western blot, or PBS plus 1% BSA for IHC) - Antibody - Blocking (immunizing) peptide - Two tubes - Two identical samples (e.g. a Western blot with two identical lanes, cut in half; two slides containing the cells of interest; etc) Method 1. Determine the optimal concentration of antibody that consistently gives a positive result in your particular protocol. Using that concentration, determine how much antibody you will need for two experiments. a. For example, an antibody is being used successfully in Western blot at 0.5 ug/ml. You will need 2 ml of antibody solution to stain one strip of a Western blot. Thus, you would use 1 ug of antibody in 2 ml buffer for each strip. 2. Dilute the necessary amount of antibody in blocking buffer to the final volume needed for the two experiments. Divide this equally into two tubes. 3. In the first tube, labeled locked, add the blocking peptide to a final concentration of 1 ug/ml (2 ug total peptide in this example). In the second tube, labeled Control, add an equivalent amount of buffer. 4. Incubate both tubes, with agitation, at room temperature for 30 minutes, or overnight at 4 degrees C. 5. Perform the staining protocol on the two identical samples, using the blocked antibody for one and the control for the other. Be careful not to mix up the strips using the blocked and control antibodies! 6. Observe the staining. The staining that disappears when using the blocked antibody is specific to the antibody. (See note i) Notes i. If more than one band disappears in Western blot by peptide/antigen competition, those bands contain the antigenic determinants and could be fragments of the full antigen or a complex containing the antigen.

CHREBP Antibody Blocking Peptide

Novus Biologicals, part of Bio-Techne | Catalog # NB400-135PEP

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Key Product Details

Conjugate

Unconjugated

Applications

Antibody Competition

View all CHREBP Proteins and Enzymes »

Product Specifications

Description

A MLXIPL antibody blocking peptide.

Application Notes

This peptide is useful as a blocking peptide for NB400-135. For further blocking peptide related protocol, click here.

Specificity

This peptide is specific for NB400-135 only.

Protein / Peptide Type

Antibody Blocking Peptide

Reviewed Applications

Read 1 review rated 4 using NB400-135PEP in the following applications:

Western Blot (1 Review)

Formulation, Preparation and Storage

NB400-135PEP

Formulation	Peptide dissolved in dH2O. Contains no BSA.
Preservative	No Preservative
Concentration	1.0 mg/ml
Shipping	The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at -80C. Avoid freeze-thaw cycles.

Background: CHREBP

ChREBP is a transcription factor involved in activating genes that encode enzymes of fatty acid biosynthesis in liver and adipose. The ChREBP protein binds and activates the carbohydrate response element (ChoRE) motifs in the promoters of triglyceride synthesis genes. ChREBP may also play roles in lipid metabolism, obesity, and type II diabetes.

The CHREBP protein is missing in the multisystem developmental disorder, Williams-Beuren syndrome. CHREBP antibodies are useful tools for studying lipid and fatty acid metabolism.

Alternate Names

BHLHD14, CHREBPcarbohydrate-responsive element-binding protein, Class D basic helix-loop-helix protein 14, MIOWS basic-helix-loop-helix leucine zipper protein, MLX interacting protein-like, MLX interactor, MLX-interacting protein-like, MONDOBWilliams-Beuren syndrome chromosomal region 14 protein, WBSCR14Williams-Beuren syndrome chromosome region 14 protein 1, Williams Beuren syndrome chromosome region 14, Williams-Beuren syndrome chromosome region 14 protein 2, WS-bHLHbHLHd14carbohydrate response element binding protein

Gene Symbol

MLXIPL

Additional CHREBP Products

Product Documents for CHREBP Antibody Blocking Peptide

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COA

Product Specific Notices for CHREBP Antibody Blocking Peptide

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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