Is mouse CNTF species specific?

Name: Recombinant Mouse CNTF Protein
Brand: Novus Biologicals
SKU: NBP2-35168

With regard to our mouse CNTF protein NBP2-35168, this particular protein is made to the mouse CNTF sequence. The functionality of this protein has only been investigated as a mouse protein. We have not yet tested this protein with use in rat samples or with rat specific antibodies. In general, the rat and mouse sequences for CNTF are very similar. Given an antibody made specifically to the mouse sequence, it is likely to cross react with the rat protein. However, with a protein product, there can be marked differences in the folding and activity of the protein that would prohibit it from being used in a species other than intended.

Recombinant Mouse CNTF Protein

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-35168

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Product Specifications
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Source

E. coli

Conjugate

Unconjugated

Applications

Bioactivity, SDS-PAGE

View all CNTF Proteins and Enzymes »

Product Specifications

Description

A single non-glycosylated polypeptide chain containing 197 amino acids corresponding to CNTF Source: E. coli

Uniprot ID: P51642

Amino Acid Sequence: AFAEQSPLTL HRRDLCSRSI WLARKIRSDL TALMESYVKH QGLNKNISLD SVDGVPVAST DRWSEMTEAE RLQENLQAYR TFQGMLTKLL EDQRVHFTPT EGDFHQAIHT LTLQVSAFAY QLEELMALLE QKVPEKEADG MPVTIGDGGL FEKKLWGLKV LQELSQWTVR SIHDLRVISS HHMGISAHES HYGAKQM

Purity

>95%, by SDS-PAGE and HPLC

Endotoxin Level

Less than 1 EU/ug of CNTF as determined by LAL method.

Predicted Molecular Mass

22.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

CNTF Protein is fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using human TF-1 cells is less than 30 ng/ml, corresponding to a specific activity of > 3.3 x 10^4 IU/mg.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Mouse CNTF Protein

SDS-PAGE: Recombinant Mouse CNTF Protein [NBP2-35168]

SDS-Page: Mouse CNTF Protein [NBP2-35168]

Formulation, Preparation and Storage

NBP2-35168

Formulation	Lyophilized from a 0.2 um filtered concentrated solution in 20 mM PB, pH 7.4, 150 mM NaCl, 5 % Trehalose, 0.02% Tween-20.
Preservative	No Preservative
Concentration	Lyoph
Reconstitution	Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL. Apportion stock solutions into working aliquots and store at
Format	Carrier-Free
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.

Background: CNTF

Ciliary neurotrophic factor (CNTF) is a polypeptide hormone whose actions appear to be restricted to the nervous system where it promotes neurotransmitter synthesis and neurite outgrowth in certain neuronal populations. CNTF was initially identified as a trophic factor for embryonic chick ciliary parasympathetic neurons in culture. Furthermore, the protein is also a potent survival factor for neurons and oligodendrocytes and may be relevant in reducing tissue destruction during inflammatory attacks. In addition, CNTF is useful for treatment of motor neuron disease and it could reduce food intake without causing hunger or stress. Recombinant murine CNTF containing 197 amino acids and it shares 82 % and 95 % a.a. sequence identity with human and rat CNTF.

Long Name

Ciliary Neurotrophic Factor

Alternate Names

ciliary neurotrophic factor, HCNTF

Gene Symbol

CNTF

Additional CNTF Products

Product Documents for Recombinant Mouse CNTF Protein

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COA

Product Specific Notices for Recombinant Mouse CNTF Protein

This lyophilized preparation is stable at 2-8 degrees C, but should be kept at -20 degrees C for long term storage, preferably desiccated. Upon reconstitution, the preparation is most stable at -20 to -80 degrees C, and can be stored for one week at 2-8 degrees C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 degrees C to -80 degrees C. Avoid repeated freeze/thaw cycles.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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