Collagen I Native Protein
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-97266
Key Product Details
Accession #
Conjugate
Unconjugated
Applications
ELISA, Immunohistochemistry, SDS-PAGE, Western Blot
Product Specifications
Description
Native Human Collagen I (COL1A1 & COL1A2 subunits)
Source: Human placenta (Accession #:P02452, P08123)
Store vial at 4C prior to opening. This product is stable at 4C as an undiluted liquid. Dilute only prior to immediate use.
No test method can provide total assurance that the hepatitis B virus, hepatitis C virus, human immunodeficiency virus, or any other infectious agents are absent. Thus, all blood products, including purified proteins derived from human blood sources, should be handled at Biosafety Level 2 as recommended by the CDC\NIH manual entitled Biosafety in Microbiological and Biomedical Laboratories for potentially infectious human serum, blood specimens or proteins derived from same. Source material for the human blood product supplied to your facility has been tested for the detection of HIV antibody, Hepatitis B surface antigen, antibody to Hepatitis C, HIV 1 antigen(s), antibody to HTLV - I/II, and syphilis by FDA guidelines. All units were found to be non-reactive/negative for these tests. All human blood source material is collected in FDA licensed centers and is tested with FDA approved test kits.
Source: Human placenta (Accession #:P02452, P08123)
Store vial at 4C prior to opening. This product is stable at 4C as an undiluted liquid. Dilute only prior to immediate use.
No test method can provide total assurance that the hepatitis B virus, hepatitis C virus, human immunodeficiency virus, or any other infectious agents are absent. Thus, all blood products, including purified proteins derived from human blood sources, should be handled at Biosafety Level 2 as recommended by the CDC\NIH manual entitled Biosafety in Microbiological and Biomedical Laboratories for potentially infectious human serum, blood specimens or proteins derived from same. Source material for the human blood product supplied to your facility has been tested for the detection of HIV antibody, Hepatitis B surface antigen, antibody to Hepatitis C, HIV 1 antigen(s), antibody to HTLV - I/II, and syphilis by FDA guidelines. All units were found to be non-reactive/negative for these tests. All human blood source material is collected in FDA licensed centers and is tested with FDA approved test kits.
Purity
Multi-step
Applications
ELISA (1:10000)
Immunohistochemistry (1:100-1:500)
Western Blot (1:1000)
Immunohistochemistry (1:100-1:500)
Western Blot (1:1000)
Application Notes
This product has been tested in SDS-PAGE. This product is suitable for western blot, ELISA, and 2D culture. Collagen type I is recognized by type specific anti-collagen antibodies that recognize a native three-dimensional structure.
Protein / Peptide Type
Native Protein
Scientific Data Images for Collagen I Native Protein
![Western Blot: Collagen I Native Protein [NBP1-97266]](https://resources.bio-techne.com/images/products/Collagen-I-Native-Protein-Western-Blot-NBP1-97266-img0002.jpg "Western Blot: Collagen I Native Protein [NBP1-97266]")
Western Blot: Collagen I Native Protein [NBP1-97266]
Western Blot: Collagen I Native Protein [NBP1-97266] - Western blot of Human Collagen I. Lane 1: Human Collagen 1. Lane 2: None. Load: 50 ng per lane. Primary antibody: Collagen I antibody at 1:1,000 overnight at 4C. Secondary antibody: DyLight 649 rabbit secondary antibody at 1:20,000 for 30 min at RT. Block for 30 min at RT. Predicted/Observed molecular weight: 139 & 130 kDa, 139 & 130 kDa for Collagen I. Other Band(s): Collagen I splice variants and isoforms.![SDS-PAGE: Collagen I Native Protein [NBP1-97266]](https://resources.bio-techne.com/images/products/Collagen I Native Protein-SDS-Page-NBP1-97266-img0005.jpg "SDS-PAGE: Collagen I Native Protein [NBP1-97266]")
SDS-PAGE: Collagen I Native Protein [NBP1-97266]
SDS-Page: Collagen I Native Protein [NBP1-97266] - SDS-PAGE Results of Human Collagen I. Lane 1: Human Collagen I Reduced (5.0ug). Lane 2: Opal Prestained Molecular Weight Marker. Lane 3: Human Collagen I Non-Reduced (5.0ug). 7.5% Gel, Coomassie Stained. Observed MW: Type I trimer, Type I dimer a1, a2, Type I monomer a1, a2.Formulation, Preparation and Storage
NBP1-97266
| Preparation Method | Prepared from human placenta and is chromatographically and immunologically pure. This product is free from other collagens, human serum proteins and non-collagen extracellular matrix proteins. |
| Formulation | 0.5 M Acetic Acid |
| Preservative | 0.01% Sodium Azide |
| Concentration | Please see the vial label for concentration. If unlisted please contact technical services. |
| Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Store at 4C. Do not freeze. |
Background: Collagen I
A variety of disease pathologies have been associated with mutations in or disruptions in collagen type I. Osteogenesis imperfecta, also known as brittle bone disease, is caused by autosomal dominant mutations in Collagen type I encoding genes and characterized by fragile bones and skeletal deformities (6). Ehlers-Danlos syndromes (EDS) is another disorder that affects soft connective tissue and its various clinical subtypes are associated with different mutations in Collagen I (7).
Alternative names for Collagen I includes alpha-1 type I collagen, alpha1(I) procollagen, CAFYD, COL1A1, collagen alpha 1 chain type I, collagen alpha-1(I) chain, EDSARTH1, EDSC, OI1, OI2, OI3, OI4, pro-alpha-1 collagen type 1, type I proalpha 1, and type I procollagen alpha 1 chain.
References
1. Chowdhury, S. R., Mh Busra, M. F., Lokanathan, Y., Ng, M. H., Law, J. X., Cletus, U. C., & Binti Haji Idrus, R. (2018). Collagen Type I: A Versatile Biomaterial. Advances in experimental medicine and biology. https://doi.org/10.1007/978-981-13-0947-2_21
2. Kisling, A., Lust, R. M., & Katwa, L. C. (2019). What is the role of peptide fragments of collagen I and IV in health and disease?. Life sciences. https://doi.org/10.1016/j.lfs.2019.04.042
3. Kwon, D., Kang, G. S., Han, D. K., Park, K., Kim, J. H., & Lee, S. H. (2014). Establishment and characterization of human engineered cells stably expressing large extracellular matrix proteins. Archives of pharmacal research. https://doi.org/10.1007/s12272-013-0294-0
4. Antoine, E. E., Vlachos, P. P., & Rylander, M. N. (2014). Review of collagen I hydrogels for bioengineered tissue microenvironments: characterization of mechanics, structure, and transport. Tissue engineering. Part B, Reviews. https://doi.org/10.1089/ten.TEB.2014.0086
5. Leon-Lopez, A., Morales-Penaloza, A., Martinez-Juarez, V. M., Vargas-Torres, A., Zeugolis, D. I., & Aguirre-alvarez, G. (2019). Hydrolyzed Collagen-Sources and Applications. Molecules (Basel, Switzerland). https://doi.org/10.3390/molecules24224031
6. Marini, J. C., Forlino, A., Bachinger, H. P., Bishop, N. J., Byers, P. H., Paepe, A., Fassier, F., Fratzl-Zelman, N., Kozloff, K. M., Krakow, D., Montpetit, K., & Semler, O. (2017). Osteogenesis imperfecta. Nature reviews. Disease primers. https://doi.org/10.1038/nrdp.2017.52
7. Callewaert, B., Malfait, F., Loeys, B., & De Paepe, A. (2008). Ehlers-Danlos syndromes and Marfan syndrome. Best practice & research. Clinical rheumatology, 22(1), 165-189. https://doi.org/10.1016/j.berh.2007.12.005
Alternate Names
Alpha-1 type I collagen, alpha1(I) procollagen, CAFYD, COL1A1, Collagen 1, collagen alpha 1 chain type I, collagen alpha-1(I) chain, collagen alpha-1(I) chain preproprotein, collagen of skin, tendon and bone, alpha-1 chain, collagen, type I, alpha 1, EDSARTH1, EDSC, OI1, OI2, OI3, OI4, pro-alpha-1 collagen type 1, type I proalpha 1, Type I Procollagen Alpha 1 Chain
Gene Symbol
COL1A1
UniProt
Additional Collagen I Products
Product Documents for Collagen I Native Protein
Product Specific Notices for Collagen I Native Protein
This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.
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