Skip to main content

Collagen IV Native Protein

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-97268

Novus Biologicals, part of Bio-Techne
Catalog #
Availability
Size / Price
Qty
Loading...
NBP1-97268

Key Product Details

Accession #

Conjugate

Unconjugated

Applications

ELISA, Immunohistochemistry, SDS-PAGE, Western Blot

Product Specifications

Description

Native Human Collagen IV

Source: Human placenta (Accession #:CAA00084.1)

Store vial at 4C prior to opening. This product is stable at 4C as an undiluted liquid. Dilute only prior to immediate use.

No test method can provide total assurance that the hepatitis B virus, hepatitis C virus, human immunodeficiency virus, or any other infectious agents are absent. Thus, all blood products, including purified proteins derived from human blood sources, should be handled at Biosafety Level 2 as recommended by the CDC\NIH manual entitled Biosafety in Microbiological and Biomedical Laboratories for potentially infectious human serum, blood specimens or proteins derived from same. Source material for the human blood product supplied to your facility has been tested for the detection of HIV antibody, Hepatitis B surface antigen, antibody to Hepatitis C, HIV 1 antigen(s), antibody to HTLV - I/II, and syphilis by FDA guidelines. All units were found to be non-reactive/negative for these tests. All human blood source material is collected in FDA licensed centers and is tested with FDA approved test kits.

Purity

Chromatography

Applications

ELISA (1:10000)
Immunohistochemistry (1:100-1:500)
Western Blot (1:1000)

Application Notes

This product has been tested in SDS-Page and can be used as a control or standard in indirect trapping ELISA for quantitation of antigen using a standard curve, for immunoprecipitation and for other immunological assays as optimized by the user. Specific conditions should be optimized by user.

Protein / Peptide Type

Native Protein

Scientific Data Images for Collagen IV Native Protein

SDS-PAGE: Collagen IV Native Protein [NBP1-97268]

SDS-PAGE: Collagen IV Native Protein [NBP1-97268]

SDS-Page: Collagen IV Native Protein [NBP1-97268] - Human Collagen Type IV. Lane 1: Human Collagen Type IV Reduced (5 ug). Lane 2: Prestained Molecular Weight Protein. Lane 3: Human Collagen Type IV Non-Reduced (5 ug). 4-20% Gel, Coomassie Stained. Observed MW: Type IV a1, a2, a3 bands.

Formulation, Preparation and Storage

NBP1-97268
Preparation Method Prepared from human placenta and is chromatographically and immunologically pure. This product is free from other collagens, human serum proteins and non-collagen extracellular matrix proteins.
Formulation 0.5 M Acetic Acid
Preservative 0.01% Sodium Azide
Concentration Please see the vial label for concentration. If unlisted please contact technical services.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at 4C. Do not freeze.

Background: Collagen IV

Collagen type IV is a type of collagen found primarily in the basal lamina. The type IV collagen C4 domain at the C-terminus is not removed in post-translational processing, and the fibers link head-to-head, rather than in parallel. Also, collagen type IV lacks the regular glycine in every third residue necessary for the tight, collagen helix. This makes the overall arrangement more sloppy with kinks. These two features cause collagen IV to form in a sheet, the form of the basal lamina. There are six human genes associated with collagen IV: COL4A1, COL4A2, COL4A3, COL4A4, COL4A5 and COL4A6.

Alternate Names

BSVD;BSVD1;COL4A1s;Collagen alpha-1(IV) chain;PADMAL;RATOR

Gene Symbol

COL4A1

UniProt

Additional Collagen IV Products

Product Documents for Collagen IV Native Protein

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Collagen IV Native Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

Loading...
Loading...
Loading...
Loading...
Loading...