Collagen V Native Protein

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-97246

Novus Biologicals, part of Bio-Techne
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Key Product Details

Conjugate

Unconjugated

Applications

ELISA, Immunohistochemistry, SDS-PAGE, Western Blot

View all Collagen V Proteins and Enzymes »

Product Specifications

Description

Native Bovine Collagen V

Source: Bovine Placenta (Accession #:DAA32356.1)

Store vial at 4C prior to opening. This product is stable at 4C as an undiluted liquid. Dilute only prior to immediate use.

Purity

Chromatography

Applications

ELISA (1:10000)
Immunohistochemistry (1:100-1:500)
Western Blot (1:1000)

Application Notes

This product has been tested in SDS-Page and can be used as a control or standard in indirect trapping ELISA for quantitation of antigen using a standard curve, for immunoprecipitation and for other immunological assays as optimized by the user. Specific conditions should be optimized by user.

Protein / Peptide Type

Native Protein

Scientific Data Images for Collagen V Native Protein

SDS-PAGE: Collagen V Native Protein [NBP1-97246]

SDS-PAGE: Collagen V Native Protein [NBP1-97246]

SDS-Page: Collagen V Native Protein [NBP1-97246] - SDS-PAGE of Collagen V Native protein. Lane 1: Collagen V Native protein Reduced (5.0ug). Lane 2: Opal Prestained Molecular Weight Marker. Lane 3: Collagen V Native protein Non-Reduced (5.0ug). Predicted MW: >95kDa.Observed MW: alpha1 and alpha2 bands.4-20% Gel Coomassie Stained.

Formulation, Preparation and Storage

NBP1-97246
Preparation Method Prepared from Bovine Placenta and is chromatographically and immunologically pure.
Formulation 0.5 M Acetic Acid
Preservative 0.01% Sodium Azide
Concentration Please see the vial label for concentration. If unlisted please contact technical services.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at 4C. Do not freeze.

Background: Collagen V

In muscle tissue, collagen serves as a major component of the endomysium. Collagen constitutes one to two percent of muscle tissue, and accounts for 6% of the weight of strong, tendinous muscles. A collagen may be defined as a protein containing sizable domain(s) of triple-helical conformation. Type IV collagen is a major macromolecular constituent of basement membranes and can be readily isolated from basement-membrane-rich tissues or highly vascularized tissues such as the placental villi. This collagen appears to be largely restricted to structures identifiable as basement membranes. In contrast, type VI collagen appears to be prevalent in several tissues even though it has been isolated largely from placental villi preparations. The extent to which type VII and VIII collagens are distributed is not known.

Alternate Names

alpha 1 type V collagen, Collagen 5, collagen alpha-1(V) chain, collagen, type V, alpha 1, Collagen-5, EC 2.7.7.6, EC 6.1.1

Gene Symbol

COL5A1

Additional Collagen V Products

Product Documents for Collagen V Native Protein

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Collagen V Native Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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