Is rhCRYGD soluble in PBS?

Name: Recombinant Human CRYGD His Protein
Brand: Novus Biologicals
SKU: NBP1-72428

This product comes in liquid in 20mM Tris-HCl buffer (pH 8.0) containing 5mM DTT, 10% glycerol, 200mM NaCl.

Recombinant Human CRYGD His Protein

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-72428

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Product Datasheet / COA

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Product Specifications
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Source

E. coli

Tag

His

Conjugate

Unconjugated

Applications

SDS-PAGE

View all CRYGD Proteins and Enzymes »

Product Specifications

Description

A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-174 of Human CRYGD

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGKITLYEDR GFQGRHYECS SDHPNLQPYL SRCNSARVDS GCWMLYEQPN YSGLQYFLRR GDYADHQQWM GLSDSVRSCR LIPHSGSHRI RLYEREDYRG QMIEFTEDCS CLQDRFRFNE IHSLNVLEGS WVLYELSNYR GRQYLLMPGD YRRYQDWGAT NARVGSLRRV IDFS

Purity

>95%, by SDS-PAGE

Predicted Molecular Mass

22.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human CRYGD His Protein

SDS-PAGE: Recombinant Human CRYGD His Protein [NBP1-72428]

SDS-Page: Recombinant Human CRYGD Protein [NBP1-72428] - 3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.

Formulation, Preparation and Storage

NBP1-72428

Formulation	20 mM Tris-HCl buffer (pH 8.0), 5mM DTT, 10% glycerol, 200 mM NaCl
Preservative	No Preservative
Concentration	1 mg/ml
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CRYGD

CRYGD belongs to the beta/gamma-crystallin family. Crystallins are the dominant structural components of the vertebrate eye lens. Mammalian lens crystallins are divided into alpha, beta, and gamma families. Gamma-crystallins have been involved in cataract formation. Defects in CRYGD are a cause of cataract autosomal dominant (ADC), cataract congenital non-nuclear polymorphic autosomal dominant (CCP), cataract congenital cerulean type 3 (CCA3) and cataract crystalline aculeiform (CACA). Recombinant human CRYGD protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Alternate Names

CACA, CCA3, CCP, CRYG4gamma crystallin 4, cry-g-D, crystallin, gamma D, Gamma-crystallin 4, gamma-crystallin D, Gamma-D-crystallin

Gene Symbol

CRYGD

Additional CRYGD Products

Product Documents for Recombinant Human CRYGD His Protein

Product Datasheets

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COA

Product Specific Notices for Recombinant Human CRYGD His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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