Recombinant Human CX3CL1/Fractalkine Protein

Name: Recombinant Human CX3CL1/Fractalkine Protein
Brand: Novus Biologicals
SKU: NBP2-34874

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-34874

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Product Specifications
Scientific Data
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Key Product Details

Source

E. coli

Accession #

P78423

Conjugate

Unconjugated

Applications

Bioactivity, SDS-PAGE

View all CX3CL1/Fractalkine Proteins and Enzymes »

Product Specifications

Description

A single non-glycosylated polypeptide chain containing 76 amino acids and comprises only the chemokine domain of CX3CL1/Fractalkine Source: E. coli

Uniprot ID: P78423

Amino Acid Sequence: QHHGVTKCNI TCSKMTSKIP VALLIHYQQN QASCGKRAII LETRQHRLFC ADPKEQWVKD AMQHLDRQAA ALTRNG

This lyophilized preparation is stable for 12 months from date of receipt at -20 to -70 degrees C, preferably desiccated. Upon reconstitution, the preparation can be stored for 1 month at 2-8 degrees C under sterile conditions, and for 3 months at -20 degrees C to -70 degrees C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Stock solutions should be aliquoted and stored at < -20 degrees C. Further dilutions should be made in appropriate buffered solutions

Purity

> 97 % pure by SDS-PAGE and HPLC

Endotoxin Level

Less than 1 EU/ug of CX3CL1/Fractalkine as determined by LAL method.

Predicted Molecular Mass

8.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

CX3CL1 Protein is fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human T-lymphocytes is in a concentration of 5.0-10 ng/ml.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human CX3CL1/Fractalkine Protein

SDS-PAGE: Recombinant Human CX3CL1/Fractalkine Protein [NBP2-34874]

SDS-Page: Human CX3CL1/Fractalkine Protein [NBP2-34874]

Formulation, Preparation and Storage

NBP2-34874

Formulation	Lyophilized from a 0.2 um filtered concentrated solution in 20 mM PB, pH 7.4, 50 mM NaCl.
Preservative	No Preservative
Concentration	Lyoph
Reconstitution	Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL. Apportion stock solutions into working aliquots and store at
Format	Carrier-Free
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.

Background: CX3CL1/Fractalkine

CX3CL1 recently identified through bioinformatics is the only known member of the CX3C chemokine family and it is also commonly known under the names fractalkine (in humans) and neurotactin (in mice). Unlike other known chemokines, CX3CL1 is a type 1 membrane protein containing a chemokine domain tethered on a long mucinlike talk. The soluble form of CX3CL1 is chemotactic for T-cells and monocytes, but not for neutrophils. In addition, it may play a role in regulating leukocyte adhesion and migration processes at the endothelium. Recombinant Human CX3CL1 which is a single non-glycosylated polypeptide chains contains 76 amino acids and it shares approximately 78 % and 83 % amino acid sequence homology with the murine and rat protein.

Alternate Names

FKN, Fractalkine, Neurotactin, Fractalkine/CX3CL1

Gene Symbol

CX3CL1

UniProt