Recombinant Rat CX3CL1/Fractalkine Protein

Name: Recombinant Rat CX3CL1/Fractalkine Protein
Brand: Novus Biologicals
SKU: NBP2-35198

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-35198

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Product Specifications
Scientific Data
Preparation & Storage
Background
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Specific Notices

Key Product Details

Source

E. coli

Accession #

O55145

Conjugate

Unconjugated

Applications

Bioactivity, SDS-PAGE

View all CX3CL1/Fractalkine Proteins and Enzymes »

Product Specifications

Description

A single non-glycosylated polypeptide chain containing 76 amino acids and comprises only the chemokine domain of CX3CL1/Fractalkine Source: E. coli

Uniprot ID: O55145

Amino Acid Sequence: QHLGMTKCNI TCHKMTSPIP VTLLIHYQLN QESCGKRAII LETRQHRHFC ADPKEKWVQD AMKHLDHQTA ALTRNG

This lyophilized preparation is stable at 2-8 degrees C, but should be kept at -20 degrees C for long term storage, preferably desiccated. Upon reconstitution, the preparation is most stable at -20 to -80 degrees C, and can be stored for one week at 2-8 degrees C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 degrees C to -80 degrees C. Avoid repeated freeze/thaw cycles.

Purity

>95%, by SDS-PAGE and HPLC

Endotoxin Level

Less than 1 EU/ug of CX3CL1/Fractalkine as determined by LAL method.

Predicted Molecular Mass

8.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

CX3CL1 Protein is fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human monocytes is in a concentration of 5.0-10 ng/ml.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Rat CX3CL1/Fractalkine Protein

SDS-PAGE: Recombinant Rat CX3CL1/Fractalkine Protein [NBP2-35198]

SDS-Page: Rat CX3CL1/Fractalkine Protein [NBP2-35198]

Formulation, Preparation and Storage

NBP2-35198

Formulation	Lyophilized from a 0.2 um filtered concentrated solution in 2x PBS, pH 7.4.
Preservative	No Preservative
Concentration	Lyoph
Reconstitution	Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL. Apportion stock solutions into working aliquots and store at
Format	Carrier-Free
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.

Background: CX3CL1/Fractalkine

CX3CL1 recently identified through bioinformatics is the only known member of the CX3C chemokine family and it is also commonly known under the names fractalkine (in humans) and neurotactin (in mice). Unlike other known chemokines, CX3CL1 is a type 1 membrane protein containing a chemokine domain tethered on a long mucinlike talk. The soluble form of CX3CL1 is chemotactic for T-cells and monocytes, but not for neutrophils. In addition, it may play a role in regulating leukocyte adhesion and migration processes at the endothelium. Recombinant Rat CX3CL1 contains 76 amino acids and it shares approximately 86 % and 83 % amino acid sequence homology with the murine and human protein.

Alternate Names

FKN, Fractalkine, Neurotactin, Fractalkine/CX3CL1

Gene Symbol

CX3CL1

UniProt