Recombinant Mouse CX3CL1/Fractalkine Protein

Name: Recombinant Mouse CX3CL1/Fractalkine Protein
Brand: Novus Biologicals
SKU: NBP2-35038

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-35038

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Product Specifications
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Background
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Key Product Details

Source

E. coli

Accession #

O35188

Conjugate

Unconjugated

Applications

Bioactivity, SDS-PAGE

View all CX3CL1/Fractalkine Proteins and Enzymes »

Product Specifications

Description

A single non-glycosylated polypeptide chain containing 76 amino acids and comprises only the chemokine domain of CX3CL1/Fractalkine c Source: E. coli

Uniprot ID: O35188

Amino Acid Sequence: QHLGMTKCEI MCGKMTSRIP VALLIRYQLN QESCGKRAIV LETTQHRRFC ADPKEKWVQD AMKHLDHQAA ALTKNG

This lyophilized preparation is stable at 2-8 degrees C, but should be kept at -20 degrees C for long term storage, preferably desiccated. Upon reconstitution, the preparation is most stable at -20 to -80 degrees C, and can be stored for one week at 2-8 degrees C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 degrees C to -80 degrees C. Avoid repeated freeze/thaw cycles.

Purity

> 97 % pure by SDS-PAGE and HPLC

Endotoxin Level

Less than 0.1 EU/ug of CX3CL1/Fractalkine as determined by LAL method.

Predicted Molecular Mass

8.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

CX3CL1 Protein is fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using human peripheral blood lymphocytes (PBL) is less than 0.5 ug/ml, corresponding to a specific activity of > 2000 IU/mg.

Protein / Peptide Type

Recombinant Protein

Formulation, Preparation and Storage

NBP2-35038

Formulation	Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.4.
Preservative	No Preservative
Concentration	Lyoph
Reconstitution	Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL. Apportion stock solutions into working aliquots and store at
Format	Carrier-Free
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.

Background: CX3CL1/Fractalkine

CX3CL1 recently identified through bioinformatics is the only known member of the CX3C chemokine family and it is also commonly known under the names fractalkine (in humans) and neurotactin (in mice). Unlike other known chemokines, CX3CL1 is a type 1 membrane protein containing a chemokine domain tethered on a long mucinlike talk. The soluble form of CX3CL1 is chemotactic for T-cells and monocytes, but not for neutrophils. CX3CL1 may play a role in regulating leukocyte adhesion and migration processes at the endothelium. Mouse CX3CL1 shares 85 % and 78 % a.a. sequence identity with rat and human CX3CL1, respectively, within the chemokine domain, but lower sequence identity within other domains.

Alternate Names

FKN, Fractalkine, Neurotactin, Fractalkine/CX3CL1

Gene Symbol

CX3CL1

UniProt