Recombinant Mouse CXCL1/GRO alpha/KC/CINC-1 Protein

Name: Recombinant Mouse CXCL1/GRO alpha/KC/CINC-1 Protein
Brand: Novus Biologicals
SKU: NBP2-35147

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-35147

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Product Specifications
Scientific Data
Preparation & Storage
Background
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Specific Notices

Key Product Details

Source

E. coli

Accession #

P12850

Conjugate

Unconjugated

Applications

Bioactivity, SDS-PAGE

View all CXCL1/GRO alpha/KC/CINC-1 Proteins and Enzymes »

Product Specifications

Description

A single non-glycosylated polypeptide chain containing 72 amino acid residues corresponding to CXCL1/GRO alpha/KC/CINC-1 Source: E. coli

Uniprot ID: P12850

Amino Acid Sequence: APIANELRCQ CLQTMAGIHL KNIQSLKVLP SGPHCTQTEV IATLKNGREA CLDPEAPLVQ KIVQKMLKGV PK

This lyophilized preparation is stable at 2-8 degrees C, but should be kept at -20 degrees C for long term storage, preferably desiccated. Upon reconstitution, the preparation is most stable at -20 to -80 degrees C, and can be stored for one week at 2-8 degrees C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 degrees C to -80 degrees C. Avoid repeated freeze/thaw cycles.

Purity

> 97 % pure by SDS-PAGE and HPLC

Endotoxin Level

Less than 1 EU/ug of CXCL1/GRO alpha/KC/CINC-1 as determined by LAL method.

Predicted Molecular Mass

7.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

CXCL1/GRO alpha/KC/CINC-1 protein is fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human peripheral blood neutrophils is in a concentration range of 10-100 ng/ml

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Mouse CXCL1/GRO alpha/KC/CINC-1 Protein

SDS-PAGE: Recombinant Mouse CXCL1/GRO alpha/KC/CINC-1 Protein [NBP2-35147]

SDS-Page: Mouse CXCL1 Protein [NBP2-35147]

Formulation, Preparation and Storage

NBP2-35147

Formulation	Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.4.
Preservative	No Preservative
Concentration	Lyoph
Reconstitution	Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL. Apportion stock solutions into working aliquots and store at
Format	Carrier-Free
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.

Background: CXCL1/GRO alpha/KC/CINC-1

Murine CXCL1, also known as KC, is belonging to the CXC chemokine family. It is encoded by the GRO gene now designated CXCL1. The gene for CXCL1 was initially discovered in mouse fibroblasts by plateletderived growth factor. KC is member of the intercrine alpha (chemokine C-X-C) subfamily of chemokines. It is secreted by human melanoma cells, and also expressed by macrophages, neutrophils and epithelial cells. The functional receptor for CXCL1 has been identified as CXCR2. CXCL1 has chemotactic activity for neutrophils, and plays a role in inflammation and wound healing. Amino acid sequence of murine CXCL1 is approximately 60 % identical to the human CXCL1. KC was found to be involved in monocyte arrest on atherosclerotic endothelium and may also play a pathophysiological role in Alzheimer's disease.

Alternate Names

CINC-1, CINC1, GRO alpha, KC, MGSA-alpha, KC/CXCL1

Gene Symbol

CXCL1

UniProt