Recombinant Rat CXCL1/GRO alpha/KC/CINC-1 Protein

Name: Recombinant Rat CXCL1/GRO alpha/KC/CINC-1 Protein
Brand: Novus Biologicals
SKU: NBP2-35250

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-35250

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Product Specifications
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Source

E. coli

Accession #

P14095

Conjugate

Unconjugated

Applications

Bioactivity, SDS-PAGE

View all CXCL1/GRO alpha/KC/CINC-1 Proteins and Enzymes »

Product Specifications

Description

A single, non-glycosylated polypeptide chain containing 72 amino acids corresponding to CXCL1/GRO alpha/KC/CINC-1 Source: E. coli

Uniprot ID: P14095

Amino Acid Sequence: APVANELRCQ CLQTVAGIHF KNIQSLKVMP PGPHCTQTEV IATLKNGREA CLDPEAPMVQ KIVQKMLKGV PK

Purity

> 97 % pure by SDS-PAGE and HPLC

Endotoxin Level

Less than 1 EU/ug of CXCL1/GRO alpha/KC/CINC-1 as determined by LAL method.

Predicted Molecular Mass

7.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

CXCL1 Protein is fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using rat neutrophils is in a concentration range of 10-100 ng/ml.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Rat CXCL1/GRO alpha/KC/CINC-1 Protein

HPLC: Recombinant Rat CXCL1/GRO alpha/KC/CINC-1 Protein [NBP2-35250]

HPLC: Recombinant Rat CXCL1 Protein [NBP2-35250] - Rat CXCL1 Protein [NBP2-35250]

Formulation, Preparation and Storage

NBP2-35250

Formulation	Lyophilized from a 0.2 um filtered concentrated solution in 20 mM PB, pH 7.4, 150 mM NaCl.
Preservative	No Preservative
Concentration	Lyoph
Reconstitution	Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL.
Format	Carrier-Free
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.

Background: CXCL1/GRO alpha/KC/CINC-1

Rat CXCL1, also known as CINC-1, is belonging to the CXC chemokine family. It is encoded by the GRO gene now designated CXCL1. It was originally purified from media conditioned by IL-1beta stimulated rat kidney epithelioid cells. CINC-1 is member of the intercrine alpha (chemokine C-X-C) subfamily of chemokines. CXCL1 is secreted by human melanoma cells, and also expressed by macrophages, neutrophils and epithelial cells. The functional receptor for CXCL1 has been identified as CXCR2. CXCL1 has chemotactic activity for neutrophils, and plays a role in inflammation and wound healing. Amino acid sequence of rat CXCL1 is approximately 68 % identical to the human CXCL1.

Alternate Names

CINC-1, CINC1, GRO alpha, KC, MGSA-alpha, CINC-1/CXCL1

Gene Symbol

CXCL1

UniProt