Recombinant Mouse CXCL10/IP-10/CRG-2 Protein

Name: Recombinant Mouse CXCL10/IP-10/CRG-2 Protein
Brand: Novus Biologicals
SKU: NBP2-35137

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-35137

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Product Datasheet / COA

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Product Specifications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Source

E. coli

Accession #

P17515

Conjugate

Unconjugated

Applications

Bioactivity, SDS-PAGE

View all CXCL10/IP-10/CRG-2 Proteins and Enzymes »

Product Specifications

Description

A single non-glycosylated polypeptide chain containing 77 amino acids corresponding to CXCL10/IP-10/CRG-2 Source: E. coli

Uniprot ID: P17515

Amino Acid Sequence: IPLARTVRCN CIHIDDGPVR MRAIGKLEII PASLSCPRVE IIATMKKNDE QRCLNPESKT IKNLMKAFSQ KRSKRAP

This lyophilized preparation is stable at 2-8 degrees C, but should be kept at -20 degrees C for long term storage, preferably desiccated. Upon reconstitution, the preparation is most stable at -20 to -80 degrees C, and can be stored for one week at 2-8 degrees C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 degrees C to -80 degrees C. Avoid repeated freeze/thaw cycles.

Purity

> 97 % pure by SDS-PAGE and HPLC

Endotoxin Level

Less than 1 EU/ug of CXCL10/IP-10/CRG-2 as determined by LAL method.

Predicted Molecular Mass

8.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

CXCL10/IP-10/CRG-2 protein is fully biologically active when compared to standard. The biologically active determined by a chemotaxis bioassay using human peripheral blood lymphocytes is in a concentration range of 0.1- 10.0 ng/ml in the presence of IL-2.

Protein / Peptide Type

Recombinant Protein

Formulation, Preparation and Storage

NBP2-35137

Formulation	Lyophilized from a 0.2 um filtered concentrated solution in 2 x PBS, pH 7.4.
Preservative	No Preservative
Concentration	Lyoph
Reconstitution	Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL. Apportion stock solutions into working aliquots and store at
Format	Carrier-Free
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.

Background: CXCL10/IP-10/CRG-2

CXCL10 also known as IP-10 is belonging to the CXC chemokine family. It is encoded by the CXCL10 gene, and in murine it is also named the CRG-2 gene. The gene was originally identified as an immediate early gene induced in response to macrophage activation. This chemokine elicits its effects by binding to the cell surface chemokine receptor CXCR3. CXCL10 has been shown to be a chemoattractant for activated T-lymphocytes and monocytes/macrophages. It also has other roles, such as promotion of T cell adhesion to endothelial cells, and inhibition of bone marrow colony formation and angiogenesis. Murine CXCL10 shares approximately 67 % amino acid sequence identity with human CXCL10.

Alternate Names

CRG-2, CRG2, IP-10, CXCL10, IP-10/CXCL10

Gene Symbol

CXCL10

UniProt