Recombinant Rat CXCL10/IP-10/CRG-2 Protein

Name: Recombinant Rat CXCL10/IP-10/CRG-2 Protein
Brand: Novus Biologicals
SKU: NBP2-35259

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-35259

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Product Datasheet / COA

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Product Specifications
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Source

E. coli

Accession #

P48973

Conjugate

Unconjugated

Applications

Bioactivity, SDS-PAGE

View all CXCL10/IP-10/CRG-2 Proteins and Enzymes »

Product Specifications

Description

A single non-glycosylated polypeptide chain containing 77 amino acids corresponding to CXCL10/IP-10/CRG-2 Source: E. coli

Uniprot ID: P48973

Amino Acid Sequence: IPLARTVRCT CIDFHEQPLR PRAIGKLEII PASLSCPHVE IIATMKKNNE KRCLNPESEA IKSLLKAVSQ RRSKRAP

Purity

>95%, by SDS-PAGE and HPLC

Endotoxin Level

Less than 1 EU/ug of CXCL10/IP-10/CRG-2 as determined by LAL method.

Predicted Molecular Mass

8.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

CXCL10/INP10 Protein is fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human CXCR3 transfected HEK293 cells is in a concentration range of 10-50 ng/ml.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Rat CXCL10/IP-10/CRG-2 Protein

SDS-PAGE: Recombinant Rat CXCL10/IP-10/CRG-2 Protein [NBP2-35259]

SDS-Page: Rat CXCL10/IP-10/CRG-2 Protein [NBP2-35259]

Formulation, Preparation and Storage

NBP2-35259

Formulation	Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.4.
Preservative	No Preservative
Concentration	Lyoph
Reconstitution	Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL. Apportion stock solutions into working aliquots and store at
Format	Carrier-Free
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.

Background: CXCL10/IP-10/CRG-2

CXCL10 also known as IP-10 is belonging to the CXC chemokine family. It is encoded by the CXCL10 gene, and in rat it is also named the Mob-1 gene. This gene was identified on the basis of differential expression in rat embryo fibroblasts. CXCL10 elicits its effects by binding to the cell surface chemokine receptor CXCR3. CXCL10 has been shown to be a chemoattractant for activated T-lymphocytes and monocytes/macrophages. It also has other roles, such as promotion of T cell adhesion to endothelial cells, and inhibition of bone marrow colony formation and angiogenesis. Rat CXCL10 shares approximately 68 % amino acid sequence identity with human CXCL10.

Alternate Names

CRG-2, CRG2, IP-10, CXCL10, IP-10/CXCL10

Gene Symbol

CXCL10

UniProt

P48973 (Rat)

Additional CXCL10/IP-10/CRG-2 Products

Product Documents for Recombinant Rat CXCL10/IP-10/CRG-2 Protein

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COA

Product Specific Notices for Recombinant Rat CXCL10/IP-10/CRG-2 Protein

This lyophilized preparation is stable at 2-8 degrees C, but should be kept at -20 degrees C for long term storage, preferably desiccated. Upon reconstitution, the preparation is most stable at -20 to -80 degrees C, and can be stored for one week at 2-8 degrees C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 degrees C to -80 degrees C. Avoid repeated freeze/thaw cycles.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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