Recombinant Mouse CXCL12/SDF-1 alpha Protein

Name: Recombinant Mouse CXCL12/SDF-1 alpha Protein
Brand: Novus Biologicals
SKU: NBP2-35155

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-35155

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Product Datasheet / COA

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Product Specifications
Preparation & Storage
Background
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Key Product Details

Source

E. coli

Accession #

P40224

Conjugate

Unconjugated

Applications

Bioactivity, SDS-PAGE

View all CXCL12/SDF-1 alpha Proteins and Enzymes »

Product Specifications

Description

A single non-glycosylated polypeptide chain containing 68 amino acids corresponding to CXCL12/SDF-1 alpha Source: E. coli

Uniprot ID: P40224

Amino Acid Sequence: KPVSLSYRCP CRFFESHIAR ANVKHLKILN TPNCALQIVA RLKNNNRQVC IDPKLKWIQE YLEKALNK

This lyophilized preparation is stable at 2-8 degrees C, but should be kept at -20 degrees C for long term storage, preferably desiccated. Upon reconstitution, the preparation is most stable at -20 to -80 degrees C, and can be stored for one week at 2-8 degrees C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 degrees C to -80 degrees C. Avoid repeated freeze/thaw cycles.

Purity

> 97 % pure by SDS-PAGE and HPLC

Endotoxin Level

Less than 1 EU/ug of CXCL12/SDF-1 alpha as determined by LAL method.

Predicted Molecular Mass

8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

CXCL1 Protein is fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human peripheral blood monocytes is in a concentration range of 50-100 ng/ml.

Protein / Peptide Type

Recombinant Protein

Formulation, Preparation and Storage

NBP2-35155

Formulation	Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.4.
Preservative	No Preservative
Concentration	Lyoph
Reconstitution	Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL. Apportion stock solutions into working aliquots and store at
Format	Carrier-Free
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.

Background: CXCL12/SDF-1 alpha

CXCL12 also known as SDF-1 is belonging to the CXC chemokine family. Murine CXCL12 is expressed as two isoforms that differ only in the C-terminal tail. Both SDF-1 isoforms undergo proteolytic processing of the first two N-terminal amino acids. Contrast to SDF-1beta, SDF-1alpha is shorter by four amino acids at the C-terminal tail. On the cell surface, the receptor for this chemokine is CXCR4 and syndecan4. CXCL12 is strongly chemotactic for T-lymphocytes, monocytes, but not neutrophils. SDF- 1 is highly conserved between species, murine CXCL12alpha shares approximately 93 % amino acid sequence identity with human CXCL12alpha.

Alternate Names

SDF1 alpha, SDF1a, CXCL12 alpha, SDF-1 alpha, SDF-1 alpha/CXCL12 alpha

Gene Symbol

CXCL12

UniProt