Recombinant Human CXCL12/SDF-1 gamma Protein

Name: Recombinant Human CXCL12/SDF-1 gamma Protein
Brand: Novus Biologicals
SKU: NBP2-35262

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-35262

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Product Datasheet / COA

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Product Specifications
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Source

E. coli

Accession #

P48061

Conjugate

Unconjugated

Applications

Bioactivity, SDS-PAGE

View all CXCL12/SDF-1 gamma Proteins and Enzymes »

Product Specifications

Description

A single non-glycosylated polypeptide chain containing 99 amino acid residues corresponding to CXCL12/SDF-1 gamma Source: E. coli

Uniprot ID: P48061

Amino Acid Sequence: GKPVSLSYRC PCRFFESHVA RANVKHLKIL NTPNCALQIV ARLKNNNRQV CIDPKLKWIQ EYLEKALNKG RREEKVGKKE KIGKKKRQKK RKAAQKRKN

Purity

>95%, by SDS-PAGE and HPLC

Endotoxin Level

Less than 0.1 EU/ug of CXCL12/SDF-1 gamma as determined by LAL method.

Predicted Molecular Mass

11.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

CXCL12/SDF-1 gamma protein is fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using PHA and rHuIL2 activated human peripheral blood T lymphocytes is in a concentration range of 30-100 ng/ml.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human CXCL12/SDF-1 gamma Protein

SDS-PAGE: Recombinant Human CXCL12/SDF-1 gamma Protein [NBP2-35262]

SDS-Page: Human CXCL12 gamma/SDF1 gamma Protein [NBP2-35262]

Formulation, Preparation and Storage

NBP2-35262

Formulation	Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.4.
Preservative	No Preservative
Concentration	Lyoph
Reconstitution	Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL.
Format	Carrier-Free
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.

Background: CXCL12/SDF-1 gamma

CXCL12 also known as SDF1 is belonging to the CXC chemokine family. It is encoded by the CXCL12 gene. In recently study, Human CXCL12 is expressed as six isoforms that differ only in the Cterminal tail. And all SDF1 isoforms undergo proteolytic processing of the first two Nterminal amino acids. Human SDF1gamma is synthesized as a 119 amino acid (a.a.) precursor that contains a 21 a.a. signal sequence and a 98 a.a. mature region and the SDF1gamma Cterminus binds heparin in secreted SDF1gamma, or targets the isoform to the nucleolus in the absence of a signal sequence. On the cell surface, the receptor for this chemokine is CXCR4 and syndecan4. CXCL12 is strongly chemotactic for Tlymphocytes, monocytes, but not neutrophils. CXCL12 is a very important factor in carcinogenesis and the neovascularisation linked to tumor progression.

Long Name

CXCL12/Stromal cell-Derived Factor 1

Alternate Names

SDF1 gamma, SDF1g

Gene Symbol

CXCL12

UniProt