Recombinant Rat CXCL2/GRO beta/MIP-2/CINC-3 Protein

Name: Recombinant Rat CXCL2/GRO beta/MIP-2/CINC-3 Protein
Brand: Novus Biologicals
SKU: NBP2-35286

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-35286

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Product Specifications
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Source

E. coli

Accession #

P30348

Conjugate

Unconjugated

Applications

Bioactivity, SDS-PAGE

View all CXCL2/GRO beta/MIP-2/CINC-3 Proteins and Enzymes »

Product Specifications

Description

A single, non-glycosylated polypeptide chain containing 73 amino acids corresponding to CXCL2/GRO beta/MIP-2/CINC-3 Source: E. coli

Uniprot ID: P30348

Amino Acid Sequence: VVVASELRCQ CLTTLPRVDF KNIQSLTVTP PGPHCAQTEV IATLKDGHEV CLNPEAPLVQ RIVQKILNKG KAN

This lyophilized preparation is stable at 2-8 degrees C, but should be kept at -20 degrees C for long term storage, preferably desiccated. Upon reconstitution, the preparation is most stable at -20 to -80 degrees C, and can be stored for one week at 2-8 degrees C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 degrees C to -80 degrees C. Avoid repeated freeze/thaw cycles.

Purity

> 98 % pure by SDS-PAGE and HPLC

Endotoxin Level

Less than 1 EU/ug of CXCL2/GRO beta/MIP-2/CINC-3 as determined by LAL method.

Predicted Molecular Mass

7.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

CXCL2 Protein is fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using rat neutrophils is in a concentration range of 10-100 ng/ml.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Rat CXCL2/GRO beta/MIP-2/CINC-3 Protein

HPLC: Recombinant Rat CXCL2/GRO beta/MIP-2/CINC-3 Protein [NBP2-35286]

HPLC: Recombinant Rat CXCL2/GRO beta/MIP-2/CINC-3 Protein [NBP2-35286] - Rat CXCL2 Protein [NBP2-35286]

Formulation, Preparation and Storage

NBP2-35286

Formulation	Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.4.
Preservative	No Preservative
Concentration	Lyoph
Reconstitution	Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL. Apportion stock solutions into working aliquots and store at
Format	Carrier-Free
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.

Background: CXCL2/GRO beta/MIP-2/CINC-3

Rat CXCL2 also named CINC-3, belongs to the CXC chemokine family. It is encoded by the gene CXCL2 and shares 90 % amino acid sequence with CXCL1/GRO alpha. It is member of the intercrine alpha (chemokine C-X-C) subfamily of chemokines. This chemokine is expressed by cytokine-stimulated rat alveolar macrophages and fibroblasts. The functional receptor for CXCL2 has been identified as CXCR2. CXCL2 is chemotactic for polymorphonuclear leukocytes and hematopoietic stem cells. Similar to other GRO proteins, CXCL2 is potent neutrophil attractants and activators. In addition, it is also active toward basophils. The amino acid sequence of rat CXCL2 is 88 % identical to murine CXCL2.

Alternate Names

CINC-3, CINC3, GRO beta, MGSA-beta, MIP-2, CINC-3/CXCL2

Gene Symbol

CXCL2

UniProt