Recombinant Rat CXCL3/GRO gamma/CINC-2/DCIP-1 Protein

Name: Recombinant Rat CXCL3/GRO gamma/CINC-2/DCIP-1 Protein
Brand: Novus Biologicals
SKU: NBP2-35251

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-35251

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Product Specifications
Preparation & Storage
Background
Product Documents
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Key Product Details

Source

E. coli

Accession #

Q10746

Conjugate

Unconjugated

Applications

Bioactivity, SDS-PAGE

View all CXCL3/GRO gamma/CINC-2/DCIP-1 Proteins and Enzymes »

Product Specifications

Description

A single non-glycosylated polypeptide chain containing 69 amino acids corresponding to CXCL3/GRO gamma/CINC-2/DCIP-1 Source: E. coli

Uniprot ID: Q10746

Amino Acid Sequence: RELRCQCLKT LPRVDFENIQ SLTVTPPGPH CTQTEVIATL KDGQEVCLNP QAPRLQKIIQ KLLKSDKSS

This lyophilized preparation is stable at 2-8 degrees C, but should be kept at -20 degrees C for long term storage, preferably desiccated. Upon reconstitution, the preparation is most stable at -20 to -80 degrees C, and can be stored for one week at 2-8 degrees C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 degrees C to -80 degrees C. Avoid repeated freeze/thaw cycles.

Purity

>95%, by SDS-PAGE and HPLC

Endotoxin Level

Less than 1 EU/ug of CXCL3/GRO gamma/CINC-2/DCIP-1 as determined by LAL method.

Predicted Molecular Mass

7.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

CXCL3 Protein is fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human CXCR2 transfected murine BaF3 cells is in a concentration range of 5-50 ng/ml.

Protein / Peptide Type

Recombinant Protein

Formulation, Preparation and Storage

NBP2-35251

Formulation	Lyophilized from a 0.2 um filtered concentrated solution in 20 mM PB, pH 7.4, 50 mM NaCl.
Preservative	No Preservative
Concentration	Lyoph
Reconstitution	Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL. Apportion stock solutions into working aliquots and store at
Format	Carrier-Free
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.

Background: CXCL3/GRO gamma/CINC-2/DCIP-1

CXCL3 is belonging to the CXC chemokine family, which is also known as CINC-2 in rat, DCIP-1 in murine, and GRO gamma in humans. The functional receptor for CXCL3 has been identified as CXCR2. Similar to other GRO proteins, CXCL3 is potent neutrophil attractants and activators. This chemokine were originally purified from the conditioned medium of rat granulation tissue. In rat, CINC-2 has two isoforms, known as CXCL3alpha/CINC-2alpha and CXCL3beta/CINC-2beta. CXCL3alpha differs from CXCL3beta as DKSS to PSL (98-101) at carboxy-terminal residues.

Alternate Names

CINC-2, Dcip1, Gm1960, GRO gamma, CINC-2 beta, CINC-2 beta/CXCL3, DCIP-1, DCIP-1/CXCL3

Gene Symbol

Cxcl3

UniProt