Recombinant Human CXCL4/PF4 Protein

Name: Recombinant Human CXCL4/PF4 Protein
Brand: Novus Biologicals
SKU: NBP2-35209

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-35209

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Product Datasheet / COA

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Product Specifications
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Source

E. coli

Accession #

P02776

Conjugate

Unconjugated

Applications

Bioactivity, SDS-PAGE

View all CXCL4/PF4 Proteins and Enzymes »

Product Specifications

Description

A single non-glycosylated polypeptide chain containing 70 amino acids corresponding to CXCL4/PF4 Source: E. coli

Uniprot ID: P02776

Amino Acid Sequence: EAEEDGDLQC LCVKTTSQVR PRHITSLEVI KAGPHCPTAQ LIATLKNGRK ICLDLQAPLY KKIIKKLLES

Purity

> 97 % pure by SDS-PAGE and HPLC

Endotoxin Level

Less than 1 EU/ug of CXCL4/PF4 as determined by LAL method.

Predicted Molecular Mass

7.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human fibroblasts is in a concentration of 1.0-10 ng/ml.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human CXCL4/PF4 Protein

Functional: Recombinant Human CXCL4/PF4 Protein [NBP2-35209]

Formulation, Preparation and Storage

NBP2-35209

Formulation	Lyophilized from a 0.2 um filtered concentrated solution in Acetonitrile and TFA.
Preservative	No Preservative
Concentration	Lyoph
Reconstitution	Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL.
Format	Carrier-Free
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.

Background: CXCL4/PF4

Platelet Factor-4 (PF-4) is a small cytokine belonging to the CXC chemokine family that is also known as chemokine (C-X-C motif) ligand 4 (CXCL4). The gene for human CXCL4 is located on human chromosome 4. This chemokine is released from alpha-granules of activated platelets during platelet aggregation and neutralizes the anticoagulant effect of heparin. It interacts with a splice variant of the chemokine receptor CXCR3 besides be chemotactic for neutrophils, fibroblasts and monocytes. Recombinant human CXCL4 contains 70 amino acids binds with high affinity to heparin. Specifically, Human and mouse CXCL4 share about a 60 % identity.

Alternate Names

PF4, CXCL4

Gene Symbol

PF4

UniProt