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Recombinant Bovine CXCL4/PF4 Protein

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-61353

Novus Biologicals, part of Bio-Techne
Catalog #
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NBP2-61353-100ug
NBP2-61353-10ug
NBP2-61353-1mg
NBP2-61353-500ug

Key Product Details

Source

E. coli

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Description

A single non-glycosylated polypeptide chain containing 88 amino acids corresponding to CXCL4/PF4 Source: E. coli

Uniprot ID: P02777

Amino Acid Sequence: ESSFPATFVP LPADSEGGED EDLQCVCLKT TSGINPRHIS SLEVIGAGTH CPSPQLLATK KTGRKICLDQ QRPLYKKILK KLLDGDES

Purity

>95%, by SDS-PAGE and HPLC

Endotoxin Level

Less than 0.1 EU/ug of CXCL4/PF4 as determined by LAL method.

Predicted Molecular Mass

9.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human neutrophils is in a concentration of 10-100ng/ml.

Protein / Peptide Type

Recombinant Protein

Formulation, Preparation and Storage

NBP2-61353
Formulation Lyophilized from a 0.2 um filtered concentrated solution in 20 mM PB, 500 mM NaCl, pH 7.0.
Preservative No Preservative
Concentration Lyoph
Reconstitution Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL. Apportion stock solutions into working aliquots and store at
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.

Background: CXCL4/PF4

Platelet factor-4 is a 70-amino acid protein that is released from the alpha-granules of activated platelets and binds with high affinity to heparin. Its major physiologic role appears to be neutralization of heparin-like molecules on the endothelial surface of blood vessels, thereby inhibiting local antithrombin III activity and promoting coagulation. As a strong chemoattractant for neutrophils and fibroblasts, PF4 probably has a role in inflammation and wound repair (Eisman et al., 1990 [PubMed 1695112]).[supplied by OMIM]

Alternate Names

PF4

Gene Symbol

PF4

Additional CXCL4/PF4 Products

Product Documents for Recombinant Bovine CXCL4/PF4 Protein

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Recombinant Bovine CXCL4/PF4 Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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