Recombinant Mouse CXCL4/PF4 Protein

Name: Recombinant Mouse CXCL4/PF4 Protein
Brand: Novus Biologicals
SKU: NBP2-35119

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-35119

Submit a review

Product Datasheet / COA

Catalog #

Availability

Size / Price

Qty

100% Guarantee

Product Specifications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Source

E. coli

Accession #

Q9Z126

Conjugate

Unconjugated

Applications

Bioactivity, SDS-PAGE

View all CXCL4/PF4 Proteins and Enzymes »

Product Specifications

Description

A single non-glycosylated polypeptide chain containing 76 amino acids corresponding to CXCL4/PF4 Source: E. coli

Uniprot ID: Q9Z126

Amino Acid Sequence: VTSAGPEESD GDLSCVCVKT ISSGIHLKHI TSLEVIKAGR HCAVPQLIAT LKNGRKICLD RQAPLYKKVI KKILES

Purity

> 97 % pure by SDS-PAGE and HPLC

Endotoxin Level

Less than 1 EU/ug of CXCL4/PF4 as determined by LAL method.

Predicted Molecular Mass

8.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

PF4/Platelet factor 4 Protein is fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human neutrophils is in a concentration of 10-100ng/ml.

Protein / Peptide Type

Recombinant Protein

Formulation, Preparation and Storage

NBP2-35119

Formulation	Lyophilized from a 0.2 um filtered concentrated solution in 20 mM PB, 1.5 M NaCl, pH 7.4.
Preservative	No Preservative
Concentration	Lyoph
Reconstitution	Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL.
Format	Carrier-Free
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.

Background: CXCL4/PF4

CXCL4 (PF4) is a small cytokine belonging to the CXC chemokine family and it is also known as chemokine (C-X-C motif) ligand 4 (CXCL4). This chemokine is released from alpha-granules of activated platelets during platelet aggregation and neutralizes the anticoagulant effect of heparin because it binds more strongly to heparin than to the chondroitin-4-sulfate chains of the carrier molecule. CXCL4 interacts with a splice variant of the chemokine receptor CXCR3. Recombinant mouse CXCL4 contains 76 amino acids which is a single non-glycosylated polypeptide chain. Specifically, Human and mouse CXCL4 share about a 60 % identity.

Alternate Names

PF4, CXCL4, PF-4/CXCL4

Gene Symbol

PF4

UniProt