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Recombinant Mouse CXCL4/PF4 Protein

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-35119

Novus Biologicals, part of Bio-Techne
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NBP2-35119-100ug
NBP2-35119-1mg
NBP2-35119-500ug
NBP2-35119-5ug

Key Product Details

Source

E. coli

Accession #

Conjugate

Unconjugated

Applications

Bioactivity, SDS-PAGE

Product Specifications

Description

A single non-glycosylated polypeptide chain containing 76 amino acids corresponding to CXCL4/PF4 Source: E. coli

Uniprot ID: Q9Z126

Amino Acid Sequence: VTSAGPEESD GDLSCVCVKT ISSGIHLKHI TSLEVIKAGR HCAVPQLIAT LKNGRKICLD RQAPLYKKVI KKILES

Purity

> 97 % pure by SDS-PAGE and HPLC

Endotoxin Level

Less than 1 EU/ug of CXCL4/PF4 as determined by LAL method.

Predicted Molecular Mass

8.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

PF4/Platelet factor 4 Protein is fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human neutrophils is in a concentration of 10-100ng/ml.

Protein / Peptide Type

Recombinant Protein

Formulation, Preparation and Storage

NBP2-35119
Formulation Lyophilized from a 0.2 um filtered concentrated solution in 20 mM PB, 1.5 M NaCl, pH 7.4.
Preservative No Preservative
Concentration Lyoph
Reconstitution Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL. Apportion stock solutions into working aliquots and store at
Format Carrier-Free
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.

Background: CXCL4/PF4

CXCL4 (PF4) is a small cytokine belonging to the CXC chemokine family and it is also known as chemokine (C-X-C motif) ligand 4 (CXCL4). This chemokine is released from alpha-granules of activated platelets during platelet aggregation and neutralizes the anticoagulant effect of heparin because it binds more strongly to heparin than to the chondroitin-4-sulfate chains of the carrier molecule. CXCL4 interacts with a splice variant of the chemokine receptor CXCR3. Recombinant mouse CXCL4 contains 76 amino acids which is a single non-glycosylated polypeptide chain. Specifically, Human and mouse CXCL4 share about a 60 % identity.

Alternate Names

PF4, CXCL4, PF-4/CXCL4

Gene Symbol

PF4

UniProt

Additional CXCL4/PF4 Products

Product Documents for Recombinant Mouse CXCL4/PF4 Protein

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Recombinant Mouse CXCL4/PF4 Protein

This lyophilized preparation is stable at 2-8 degrees C, but should be kept at -20 degrees C for long term storage, preferably desiccated. Upon reconstitution, the preparation is most stable at -20 to -80 degrees C, and can be stored for one week at 2-8 degrees C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 degrees C to -80 degrees C. Avoid repeated freeze/thaw cycles.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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