Recombinant Mouse CXCL5/ENA-78 Protein

Name: Recombinant Mouse CXCL5/ENA-78 Protein
Brand: Novus Biologicals
SKU: NBP2-35154

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-35154

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Product Datasheet / COA

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Product Specifications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Source

E. coli

Accession #

P50228

Conjugate

Unconjugated

Applications

Bioactivity, SDS-PAGE

View all CXCL5/ENA-78 Proteins and Enzymes »

Product Specifications

Description

A single non-glycosylated polypeptide chain containing 70 amino acids corresponding to CXCL5/ENA-78 Source: E. coli

Uniprot ID: P50228

Amino Acid Sequence: TELRCVCLTV TPKINPKLIA NLEVIPAGPQ CPTVEVIAKL KNQKEVCLDP EAPVIKKIIQ KILGSDKKKA

This lyophilized preparation is stable at 2-8 degrees C, but should be kept at -20 degrees C for long term storage, preferably desiccated. Upon reconstitution, the preparation is most stable at -20 to -80 degrees C, and can be stored for one week at 2-8 degrees C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 degrees C to -80 degrees C. Avoid repeated freeze/thaw cycles.

Purity

>95%, by SDS-PAGE and HPLC

Endotoxin Level

Less than 0.1 EU/ug of CXCL5/ENA-78 as determined by LAL method.

Predicted Molecular Mass

7.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

CXCL5 Protein is fully biologically active when compared to standard. The biological activity determined by a chemoattract bioassay using human neutrophils is in a concentration range of 10-100 ng/ml.

Protein / Peptide Type

Recombinant Protein

Formulation, Preparation and Storage

NBP2-35154

Formulation	Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.4.
Preservative	No Preservative
Concentration	Lyoph
Reconstitution	Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL. Apportion stock solutions into working aliquots and store at
Format	Carrier-Free
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.

Background: CXCL5/ENA-78

CXCL5 is a small cytokine belonging to the CXC chemokine family and it is encoded by the CXCL5 gene. It may participate in the recruitment of inflammatory cells by injured or infected tissue. CXCL5 is cleaved into the following 2 chains: GCP-2 (1-78) and GCP-2 (9-78) which attract neutrophils and are involved in neutrophil activation. Recombinant Mouse CXCL5 contains 70 amino acids and shares 57 % amino acid sequence identity with human CXCL5.

Alternate Names

CXCL5, ENA-78, ENA78, LIX/CXCL5

Gene Symbol

CXCL5

UniProt