Recombinant Rat CXCL5/ENA-78 Protein

Name: Recombinant Rat CXCL5/ENA-78 Protein
Brand: Novus Biologicals
SKU: NBP2-35266

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-35266

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Product Specifications
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Source

E. coli

Accession #

P97885

Conjugate

Unconjugated

Applications

Bioactivity, SDS-PAGE

View all CXCL5/ENA-78 Proteins and Enzymes »

Product Specifications

Description

A single, non-glycosylated polypeptide chain containing 93 amino acids corresponding to CXCL5/ENA-78 Source: E. coli

Uniprot ID: P97885

Amino Acid Sequence: APFSAMVATE LRCVCLTLAP RINPKMIANL EVIPAGPHCP KVEVIAKLKN QKDNVCLDPQ APLIKKVIQK ILGSENKKTK RNALALVRSA STQ

This lyophilized preparation is stable at 2-8 degrees C, but should be kept at -20 degrees C for long term storage, preferably desiccated. Upon reconstitution, the preparation is most stable at -20 to -80 degrees C, and can be stored for one week at 2-8 degrees C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 degrees C to -80 degrees C. Avoid repeated freeze/thaw cycles.

Purity

> 97 % pure by SDS-PAGE and HPLC

Endotoxin Level

Less than 1 EU/ug of CXCL5/ENA-78 as determined by LAL method.

Predicted Molecular Mass

10.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

CXCL5 Protein is fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human peripheral blood neutrophils is in a concentration of 10-100 ng/ml.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Rat CXCL5/ENA-78 Protein

SDS-PAGE: Recombinant Rat CXCL5/ENA-78 Protein [NBP2-35266]

SDS-Page: Rat CXCL5 Protein [NBP2-35266]

Formulation, Preparation and Storage

NBP2-35266

Formulation	Lyophilized from a 0.2 um filtered concentrated solution in 2 x PBS, pH 7.4.
Preservative	No Preservative
Concentration	Lyoph
Reconstitution	Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL. Apportion stock solutions into working aliquots and store at
Format	Carrier-Free
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.

Background: CXCL5/ENA-78

CXCL5 is a small cytokine belonging to the CXC chemokine family. It is produced following stimulation of cells with the inflammatory cytokines interleukin-1 or tumor necrosis factor-alpha. The expression of CXCL5 has also been observed in eosinophils, and can be inhibited with the type II interferon IFN-gamma. Recombinant rat CXCL5 contains 93 amino acids which is a single, non-glycosylated polypeptide chain. Rat CXCL5 shares 57% amino acid sequence identity with human CXCL5.

Alternate Names

CXCL5, ENA-78, ENA78, LIX/CXCL5

Gene Symbol

CXCL5

UniProt