Recombinant Human CXCL9/MIG Protein

Name: Recombinant Human CXCL9/MIG Protein
Brand: Novus Biologicals
SKU: NBP2-35008

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-35008

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Product Specifications
Scientific Data
Preparation & Storage
Background
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Key Product Details

Source

E. coli

Accession #

Q07325

Conjugate

Unconjugated

Applications

Bioactivity, SDS-PAGE

View all CXCL9/MIG Proteins and Enzymes »

Product Specifications

Description

A single non-glycosylated polypeptide chain containing 103 amino acids corresponding to CXCL9/MIG Source: E. coli

Uniprot ID: Q07325

Amino Acid Sequence: TPVVRKGRCS CISTNQGTIH LQSLKDLKQF APSPSCEKIE IIATLKNGVQ TCLNPDSADV KELIKKWEKQ VSQKKKQKNG KKHQKKKVLK VRKSQRSRQK KTT

Purity

> 97 % pure by SDS-PAGE and HPLC

Endotoxin Level

Less than 1 EU/ug of CXCL9/MIG as determined by LAL method.

Predicted Molecular Mass

11.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

MIG Protein is fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human peripheral blood T-lymphocytes is in a concentration range of 10-100 ng/ml.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human CXCL9/MIG Protein

SDS-PAGE: Recombinant Human CXCL9/MIG Protein [NBP2-35008]

SDS-Page: Human CXCL9/MIG Protein [NBP2-35008]

Formulation, Preparation and Storage

NBP2-35008

Formulation	Lyophilized from a 0.2 um filtered concentrated solution in 20 mM PB, pH 7.4, 50 mM NaCl.
Preservative	No Preservative
Concentration	Lyoph
Reconstitution	Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL.
Format	Carrier-Free
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.

Background: CXCL9/MIG

CXCL9 is a T-cell chemoattractant induced by IFN-gamma belonging to the CXC chemokine family and it is also known as Monokine induced by gamma interferon (MIG). CXCL9 is closely related to two other CXC chemokines called CXCL10 and CXCL11 and they all elicit their chemotactic functions by interacting with the chemokine receptor CXCR3. CXCL9 is a cytokine that affects the growth, movement, or activation state of cells that participate in immune and inflammatory response and chemotactic for activated T-cells. Recombinant human CXCL9 contains 103 amino acids which is a single non-glycosylated polypeptide chain. The human CXCL9 shares 75 % and 67 % a.a. sequence identity with mouse and rat CXCL9.

Alternate Names

MIG, CXCl9, MIG/CXCL9

Gene Symbol

CXCL9

UniProt