Recombinant Mouse CXCL9/MIG Protein

Name: Recombinant Mouse CXCL9/MIG Protein
Brand: Novus Biologicals
SKU: NBP2-35191

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-35191

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Product Specifications
Scientific Data
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Background
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Key Product Details

Source

E. coli

Accession #

P18340

Conjugate

Unconjugated

Applications

Bioactivity, SDS-PAGE

View all CXCL9/MIG Proteins and Enzymes »

Product Specifications

Description

A single non-glycosylated polypeptide chain containing 105 amino acids corresponding to CXCL9/MIG Source: E. coli

Uniprot ID: P18340

Amino Acid Sequence: TLVIRNARCS CISTSRGTIH YKSLKDLKQF APSPNCNKTE IIATLKNGDQ TCLDPDSANV KKLMKEWEKK INQKKKQKRG KKHQKNMKNR KPKTPQSRRR SRKTT

This lyophilized preparation is stable at 2-8 degrees C, but should be kept at -20 degrees C for long term storage, preferably desiccated. Upon reconstitution, the preparation is most stable at -20 to -80 degrees C, and can be stored for one week at 2-8 degrees C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 degrees C to -80 degrees C. Avoid repeated freeze/thaw cycles.

Purity

>95%, by SDS-PAGE and HPLC

Endotoxin Level

Less than 1 EU/ug of CXCL9/MIG as determined by LAL method.

Predicted Molecular Mass

12.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

MIG Protein is fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human lymphocytes is in a concentration range of 0.1-1.0 ng/ml.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Mouse CXCL9/MIG Protein

SDS-PAGE: Recombinant Mouse CXCL9/MIG Protein [NBP2-35191]

SDS-Page: Mouse CXCL9/MIG Protein [NBP2-35191]

Formulation, Preparation and Storage

NBP2-35191

Formulation	Lyophilized from a 0.2 um filtered concentrated solution in 2 x PBS, pH 7.4.
Preservative	No Preservative
Concentration	Lyoph
Reconstitution	Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL. Apportion stock solutions into working aliquots and store at
Format	Carrier-Free
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.

Background: CXCL9/MIG

CXCL9 belongs to the CXC chemokine family and also known as Monokine induced by gamma interferon (MIG). It is a T-cell chemoattractant induced by IFN-gamma. CXCL9 is closely related to two other CXC chemokines called CXCL10 and CXCL11, additionally they all elicit their chemotactic functions by interacting with the chemokine receptor CXCR3. It is a cytokine that affects the growth, movement, or activation state of cells that participate in immune and inflammatory response and chemotactic for activated T-cells. Recombinant murine CXCL9 contains 105 amino acids which is a single non-glycosylated polypeptide chain. Furthermore, The murine CXCL9 shares 75 % and 88 % a.a. sequence identity with human and rat CXCL9.

Alternate Names

MIG, CXCl9, MIG/CXCL9

Gene Symbol

CXCL9

UniProt