Recombinant Human CYB5R3 His Protein

Name: Recombinant Human CYB5R3 His Protein
Brand: Novus Biologicals
SKU: NBP1-78879

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-78879

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Product Specifications
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Source

E. coli

Tag

His

Conjugate

Unconjugated

Applications

SDS-PAGE

View all CYB5R3 Proteins and Enzymes »

Product Specifications

Description

A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 27-301 of Human CYB5R3

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSHMFQRST PAITLESPDI KYPLRLIDRE IISHDTRRFR FALPSPQHIL GLPVGQHIYL SARIDGNLVV RPYTPISSDD DKGFVDLVIK VYFKDTHPKF PAGGKMSQYL ESMQIGDTIE FRGPSGLLVY QGKGKFAIRP DKKSNPIIRT VKSVGMIAGG TGITPMLQVI RAIMKDPDDH TVCHLLFANQ TEKDILLRPE LEELRNKHSA RFKLWYTLDR APEAWDYGQG FVNEEMIRDH LPPPEEEPLV LMCGPPPMIQ YACLPNLDHV GHPTERCFVF

Purity

>95%, by SDS-PAGE

Predicted Molecular Mass

34 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human CYB5R3 His Protein

SDS-PAGE: Recombinant Human CYB5R3 His Protein [NBP1-78879]

SDS-Page: Recombinant Human CYB5R3 Protein [NBP1-78879] - 3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain

Formulation, Preparation and Storage

NBP1-78879

Formulation	20 mM Tris-HCl buffer (pH 8.0), 10% glycerol, 1 mM DTT, 0.1 M NaCl
Preservative	No Preservative
Concentration	1 mg/ml
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CYB5R3

CYB5R3 is a cytochrome b5 reductasecytochrome b5 reductase, which includes a membrane-bound form in somatic cells (anchored in the endoplasmic reticulum, mitochondrial and other membranes) and a soluble form in erythrocytes. The membrane-bound form exists mainly on the cytoplasmic side of the endoplasmic reticulum and functions in desaturation and elongation of fatty acids, in cholesterol biosynthesis, and in drug metabolism. The erythrocyte form is located in a soluble fraction of circulating erythrocytes and is involved in methemoglobin reduction. The membrane-bound form has both membrane-binding and catalytic domains, while the soluble form has only the catalytic domain. Mutations in this gene cause methemoglobinemias. Recombinant human CYB5R3 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Alternate Names

B5RNADH-cytochrome b5 reductase 3, Cytochrome b5 reductase, cytochrome b5 reductase 3, DIA1NADH-cytochrome b5 reductase 3 membrane-bound form, diaphorase (NADH) (cytochrome b-5 reductase), diaphorase-1, EC 1.6.2.2, NADH-cytochrome b5 reductase 3 soluble form

Gene Symbol

CYB5R3

Additional CYB5R3 Products

Product Documents for Recombinant Human CYB5R3 His Protein

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COA

Product Specific Notices for Recombinant Human CYB5R3 His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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