Recombinant Human Cyclophilin-F His Protein
Novus Biologicals, part of Bio-Techne | Catalog # NBC1-18533
Key Product Details
Source
E. coli
Tag
His
Conjugate
Unconjugated
Applications
Bioactivity, Immunoprecipitation, SDS-PAGE, Western Blot
Product Specifications
Description
A bioactive recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 30-207 of Human Cyclophilin-F
Source: E.coli
Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MCSKGSGDPS SSSSSGNPLV YLDVDANGKP LGRVVLELKA DVVPKTAENF RALCTGEKGF GYKGSTFHRV IPSFMCQAGD FTNHNGTGGK SIYGSRFPDE NFTLKHVGPG VLSMANAGPN TNGSQFFICT IKTDWLDGKH VVFGHVKEGM DVVKKIESFG SKSGRTSKKI VITDCGQLS
Purity
>95%, by SDS-PAGE
Endotoxin Level
< 1.0 EU per 1ug of protein (determined by LAL method)
Predicted Molecular Mass
21.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Activity
Specific activity is > 900 nmol/min/mg, and is defined as the amount of enzyme that cleaves 1nmole of suc-AAPF-pNA per minute at 37C in Tris-HCl pH 8.0 using chymotrypsin.
Application Notes
Use in immunoprecipitation, and western blot reported in scientific literature (PMID 26387735)
Protein / Peptide Type
Recombinant Protein
Scientific Data Images for Recombinant Human Cyclophilin-F His Protein
![SDS-PAGE: Recombinant Human Cyclophilin-F His Protein [NBC1-18533]](https://resources.bio-techne.com/images/products/Recombinant-Human-Cyclophilin-F-Protein-SDS-Page-NBC1-18533-img0002.jpg "SDS-PAGE: Recombinant Human Cyclophilin-F His Protein [NBC1-18533]")
SDS-PAGE: Recombinant Human Cyclophilin-F His Protein [NBC1-18533]
SDS-Page: Recombinant Human Cyclophilin-F Protein [NBC1-18533] - Cyclophilin F(PPIF), 21 kDa (198aa), confirmed by MALDI-TOF with a purity of 95% by SDS - PAGEFormulation, Preparation and Storage
NBC1-18533
| Formulation | 20 mM Tris-HCl buffer (pH 7.5), 1 mM DTT, 10% glycerol |
| Preservative | No Preservative |
| Concentration | 1 mg/ml |
| Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
Background: Cyclophilin-F
Alternate Names
Cyclophilin F, CYP3FLJ90798, Cyp-D, hCyP3, MGC117207, mitochondrial, peptidyl-prolyl cis-trans isomerase, mitochondrial, peptidylprolyl isomerase F, peptidylprolyl isomerase F (cyclophilin F)
Gene Symbol
PPIF
Additional Cyclophilin-F Products
Product Documents for Recombinant Human Cyclophilin-F His Protein
Product Specific Notices for Recombinant Human Cyclophilin-F His Protein
This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.
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