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Recombinant Human DARS His Protein

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-99067

Novus Biologicals, part of Bio-Techne
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NBP1-99067-0.5mg
NBP1-99067-100ug

Key Product Details

Source

E. coli

Tag

His

Conjugate

Unconjugated

Applications

SDS-PAGE

Product Specifications

Description

A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-501 of Human DARS

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MPSASASRKS QEKPREIMDA AEDYAKERYG ISSMIQSQEK PDRVLVRVRD LTIQKADEVV WVRARVHTSR AKGKQCFLVL RQQQFNVQAL VAVGDHASKQ MVKFAANINK ESIVDVEGVV RKVNQKIGSC TQQDVELHVQ KIYVISLAEP RLPLQLDDAV RPEAEGEEEG RATVNQDTRL DNRVIDLRTS TSQAVFRLQS GICHLFRETL INKGFVEIQT PKIISAASEG GANVFTVSYF KNNAYLAQSP QLYKQMCICA DFEKVFSIGP VFRAEDSNTH RHLTEFVGLD IEMAFNYHYH EVMEEIADTM VQIFKGLQER FQTEIQTVNK QFPCEPFKFL EPTLRLEYCE ALAMLREAGV EMGDEDDLST PNEKLLGHLV KEKYDTDFYI LDKYPLAVRP FYTMPDPRNP KQSNSYDMFM RGEEILSGAQ RIHDPQLLTE RALHHGIDLE KIKAYIDSFR FGAPPHAGGG IGLERVTMLF LGLHNVRQTS MFPRDPKRLT P

Purity

>85%, by SDS-PAGE

Predicted Molecular Mass

59.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human DARS His Protein

SDS-PAGE: Recombinant Human DARS His Protein [NBP1-99067]

SDS-PAGE: Recombinant Human DARS His Protein [NBP1-99067]

SDS-Page: Recombinant Human DARS Protein [NBP1-99067] - 15% SDS-PAGE (3ug)

Formulation, Preparation and Storage

NBP1-99067
Formulation 20 mM Tris-HCl buffer (pH8.0), 40% glycerol, 0.15 M NaCl, 1 mM DTT
Preservative No Preservative
Concentration 1 mg/ml
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: DARS

DARS, also known as aspartyl-tRNA synthetase, catalyzes the specific attachment of an amino acid to its cognate tRNA in a 2 step reaction: the amino acid (AA) is first activated by ATP to form AA-AMP and then transferred to the acceptor end of the tRNA. Recombinant human DARS protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.

Alternate Names

aspartate tRNA ligase 1, cytoplasmic, Aspartate--tRNA ligase, aspartyl-tRNA synthetase, aspartyl-tRNA synthetase, cytoplasmic, AspRS, Cell proliferation-inducing gene 40 protein, cell proliferation-inducing protein 40, DKFZp781B11202, EC 6.1.1.12, MGC111579

Gene Symbol

DARS1

Additional DARS Products

Product Documents for Recombinant Human DARS His Protein

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Recombinant Human DARS His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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