Recombinant Human DCPS His Protein

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-72406

Novus Biologicals, part of Bio-Techne
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NBP1-72406-250ug
NBP1-72406-50ug
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Key Product Details

Source

E. coli

Tag

His

Conjugate

Unconjugated

Applications

SDS-PAGE

View all DCPS Proteins and Enzymes »

Product Specifications

Description

A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-337 of Human DCPS

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MADAAPQLGK RKRELDVEEA HAASTEEKEA GVGNGTCAPV RLPFSGFRLQ KVLRESARDK IIFLHGKVNE ASGDGDGEDA VVILEKTPFQ VEQVAQLLTG SPELQLQFSN DIYSTYHLFP PRQLNDVKTT VVYPATEKHL QKYLRQDLRL IRETGDDYRN ITLPHLESQS LSIQWVYNIL DKKAEADRIV FENPDPSDGF VLIPDLKWNQ QQLDDLYLIA ICHRRGIRSL RDLTPEHLPL LRNILHQGQE AILQRYRMKG DHLRVYLHYL PSYYHLHVHF TALGFEAPGS GVERAHLLAE VIENLECDPR HYQQRTLTFA LRADDPLLKL LQEAQQS

Purity

>90%, by SDS-PAGE

Predicted Molecular Mass

40.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human DCPS His Protein

SDS-PAGE: Recombinant Human DCPS His Protein [NBP1-72406]

SDS-PAGE: Recombinant Human DCPS His Protein [NBP1-72406]

SDS-Page: DCPS Protein [NBP1-72406] - 15% SDS-PAGE (3 ug protein loaded).

Formulation, Preparation and Storage

NBP1-72406
Formulation 20 mM Tris-HCl buffer (pH8.0), 20% glycerol, 1 mM DTT
Preservative No Preservative
Concentration 0.5 mg/ml
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: DCPS

DCPS, also known as scavenger mRNA-decapping enzyme, is belongs to the HIT family. It is necessary for the complete degradation of mRNAs, both in normal mRNA turnover and in nonsense-mediated mRNA decay. A scavenger decapping enzyme, DcpS was shown to hydrolyze the residual m7GpppN cap structure after the complete 3'-5' degradation of the mRNA by the exosome. DcpS releases m7GMP and is unable to cleave cap structures attached to a long RNA chain. Recombinant human DCPS protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.

Alternate Names

DCS1, DCS-1, decapping enzyme, scavenger, EC 3.-, heat shock-like protein 1, HINT5, HINT-5homolog of C. elegans 7meGMP-directed hydrolase dcs-1, Hint-related 7meGMP-directed hydrolase, Histidine triad protein member 5, HSL1, HSPC015, mRNA decapping enzyme, scavenger mRNA-decapping enzyme DcpS

Gene Symbol

DCPS

Additional DCPS Products

Product Documents for Recombinant Human DCPS His Protein

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Recombinant Human DCPS His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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