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Recombinant Mouse Desert Hedgehog C23II Protein

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-35175

Novus Biologicals, part of Bio-Techne
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NBP2-35175-100ug
NBP2-35175-1mg
NBP2-35175-5ug

Key Product Details

Source

E. coli

Accession #

Conjugate

Unconjugated

Applications

Bioactivity, SDS-PAGE

Product Specifications

Description

A single non-glycosylated polypeptide chain containing 177 amino acids corresponding to Desert Hedgehog C23II Source: E. coli

Uniprot ID: Q61488

Amino Acid Sequence: IIGPGRGPVG RRRYVRKQLV PLLYKQFVPS MPERTLGASG PAEGRVTRGS ERFRDLVPNY NPDIIFKDEE NSGADRLMTE RCKERVNALA IAVMNMWPGV RLRVTEGWDE DGHHAQDSLH YEGRALDITT SDRDRNKYGL LARLAVEAGF DWVYYESRNH IHVSVKADNS LAVRAGG

Purity

> 98 % pure by SDS-PAGE and HPLC

Endotoxin Level

Less than 0.1 EU/ug of Desert Hedgehog C23II as determined by LAL method.

Predicted Molecular Mass

20.0 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

Desert Hedgehog C23II protein is fully biologically active when compared to standard. The ED50 as determined by its ability to induce alkaline phosphatase production by murine MC3T3E1 cells is 5-20 ug/ml.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Mouse Desert Hedgehog C23II Protein

SDS-PAGE: Recombinant Mouse Desert Hedgehog C23II Protein [NBP2-35175]

SDS-PAGE: Recombinant Mouse Desert Hedgehog C23II Protein [NBP2-35175]

SDS-Page: Mouse Desert Hedgehog C23II Protein [NBP2-35175]

Formulation, Preparation and Storage

NBP2-35175
Formulation Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.4, 1 mM DTT, 0.05 % Tween-80.
Preservative No Preservative
Concentration Lyoph
Reconstitution Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL. Apportion stock solutions into working aliquots and store at
Format Carrier-Free
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.

Background: Desert Hedgehog C23II

Desert hedgehog (Dhh) belongs to the highly conserved Hedgehog family of proteins which are involved in multiple developmental processes. Hedgehogs are cleaved autocatalytically, and the N-terminal fragment remains membrane associated due to its cholesterol and palmitate modifications. Dhh binds Patched, Patched 2, and Hedgehog interacting protein (Hip), inhibiting the ability of Patched to repress Smoothened signaling. Dhh is produced by Sertoli cells and is required for testis development, Leydig cell differentiation, and spermatogenesis. It is expressed in the female by ovarian granulosa cells and the corporus luteum and is upregulated in ovarian cancer. Dhh is also expressed by Schwann cells and is upregulated following nerve injury.

Gene Symbol

DHH

UniProt

Additional Desert Hedgehog C23II Products

Product Documents for Recombinant Mouse Desert Hedgehog C23II Protein

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Recombinant Mouse Desert Hedgehog C23II Protein

This lyophilized preparation is stable at 2-8 degrees C, but should be kept at -20 degrees C for long term storage, preferably desiccated. Upon reconstitution, the preparation is most stable at -20 to -80 degrees C, and can be stored for one week at 2-8 degrees C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 degrees C to -80 degrees C. Avoid repeated freeze/thaw cycles.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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