Recombinant Human DIMT1L His Protein

Name: Recombinant Human DIMT1L His Protein
Brand: Novus Biologicals
SKU: NBP1-98909

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-98909

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Technical Data
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Product Specifications
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Source

E. coli

Tag

His

Conjugate

Unconjugated

Applications

SDS-PAGE

View all DIMT1L Proteins and Enzymes »

Product Specifications

Description

A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-313 of Human DIMT1L

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MMPKVKSGAI GRRRGRQEQR RELKSAGGLM FNTGIGQHIL KNPLIINSII DKAALRPTDV VLEVGPGTGN MTVKLLEKAK KVVACELDPR LVAELHKRVQ GTPVASKLQV LVGDVLKTDL PFFDTCVANL PYQISSPFVF KLLLHRPFFR CAILMFQREF ALRLVAKPGD KLYCRLSINT QLLARVDHLM KVGKNNFRPP PKVESSVVRI EPKNPPPPIN FQEWDGLVRI TFVRKNKTLS AAFKSSAVQQ LLEKNYRIHC SVHNIIIPED FSIADKIQQI LTSTGFSDKR ARSMDIDDFI RLLHGFNAEG IHFS

Purity

>90%, by SDS-PAGE

Predicted Molecular Mass

37.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human DIMT1L His Protein

SDS-PAGE: Recombinant Human DIMT1L His Protein [NBP1-98909]

SDS-Page: Recombinant Human DIMT1L His Protein [NBP1-98909] - 3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain

Formulation, Preparation and Storage

NBP1-98909

Formulation	20 mM Tris-HCl buffer (pH8.0), 30% glycerol, 2 mM DTT, 200 mM NaCl, 2 mM EDTA
Preservative	No Preservative
Concentration	0.25 mg/ml
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: DIMT1L

DIMT1 (DIM1 dimethyladenosine transferase 1 homolog) belongs to the methyltransferase superfamily. This enzyme is specifically dimethylates two adjacent adenosines in the loop of a conserved hairpin near the 3'-end of 18S rRNA in the 40S particle. It functions to dimethylate adjacent adenosines on the conserved hairpin loop of 18S rRNA in the 40S particle. DIMT1L is localized to the nucleolus. Recombinant human DIMT1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Alternate Names

18S rRNA (adenine(1779)-N(6)/adenine(1780)-N(6))-dimethyltransferase, DIM1, DIM1 dimethyladenosine transferase 1-like, DIM1 dimethyladenosine transferase 1-like (S. cerevisiae), DIMT1dimethyladenosine transferase, EC 2.1.1, EC 2.1.1.14, EC 2.1.1.183,18S rRNA dimethylase, HSA9761, HUSSY5, N'-adenosyl(rRNA) dimethyltransferase, probable dimethyladenosine transferase, S-adenosylmethionine-6-N'

Gene Symbol

DIMT1

Additional DIMT1L Products

Product Documents for Recombinant Human DIMT1L His Protein

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COA

Product Specific Notices for Recombinant Human DIMT1L His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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