Recombinant Human Drosha GST (N-Term) Protein

Name: Recombinant Human Drosha GST (N-Term) Protein
Brand: Novus Biologicals
SKU: H00029102-Q01

Novus Biologicals, part of Bio-Techne | Catalog # H00029102-Q01

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Product Specifications
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Source

Wheat germ

Tag

GST (N-Term)

Conjugate

Unconjugated

Applications

ELISA, Affinity Purification, Microarray, Western Blot

View all Drosha Proteins and Enzymes »

Product Specifications

Description

A recombinant protein with a N-terminal GST tag corresponding to the amino acid sequence 1099-1196 of Human Drosha

Source: Wheat Germ (in vitro)

Amino Acid Sequence: ETSPVLQKLTEFEEAIGVIFTHVRLLARAFTLRTVGFNHLTLGHNQRMEFLGDSIMQLVATEYLFIHFPDHHEGHLTLLRSSLVNNRTQAKVAEELGM

Purity

>80% by SDS-PAGE and Coomassie blue staining

Predicted Molecular Mass

36.52 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

This protein was produced in an in vitro wheat germ expression system that should preserve correct conformational folding that is necessary for biological function. While it is possible that this protein could display some level of activity, the functionality of this protein has not been explicitly measured or validated.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human Drosha GST (N-Term) Protein

SDS-PAGE: Recombinant Human Drosha GST (N-Term) Protein [H00029102-Q01]

SDS-Page: Recombinant Human Drosha Protein [H00029102-Q01] - 12.5% SDS-PAGE Stained with Coomassie Blue.

Formulation, Preparation and Storage

H00029102-Q01

Preparation Method	in vitro wheat germ expression system
Formulation	50 mM Tris-HCI, 10 mM reduced Glutathione, pH 8.0 in the elution buffer.
Preservative	No Preservative
Concentration	Please see the vial label for concentration. If unlisted please contact technical services.
Shipping	The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at -80C. Avoid freeze-thaw cycles.

Background: Drosha

Members of the ribonuclease III superfamily of double-stranded (ds) RNA-specific endoribonucleases participate in diverse RNA maturation and decay pathways in eukaryotic and prokaryotic cells (Fortin et al., 2002 [PubMed 12191433]). The RNase III Drosha is the core nuclease that executes the initiation step of microRNA (miRNA) processing in the nucleus (Lee et al., 2003 [PubMed 14508493]).[supplied by OMIM]

Alternate Names

drosha, double-stranded RNA-specific endoribonuclease, drosha, ribonuclease type III, EC 3.1.26.3, ETOHI2, nuclear RNase III Drosha, p241, Protein Drosha, putative protein p241 which interacts with transcription factor Sp1, putative ribonuclease III, RANSE3L, ribonuclease 3, Ribonuclease III, ribonuclease III, nuclear, RN3nuclear, RNase III

Gene Symbol

DROSHA

Additional Drosha Products

Product Documents for Recombinant Human Drosha GST (N-Term) Protein

Product Datasheets

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COA

SDS

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Product Specific Notices for Recombinant Human Drosha GST (N-Term) Protein

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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