Recombinant Human dUTPase His Protein

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-48598

Novus Biologicals, part of Bio-Techne
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NBP1-48598-0.5mg
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Key Product Details

Source

E. coli

Tag

His

Conjugate

Unconjugated

Applications

SDS-PAGE

View all dUTPase Proteins and Enzymes »

Product Specifications

Description

A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 70-252 of Human dUTPase

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MASTVGAAGW KGELPKAGGS PAPGPETPAI SPSKRARPAE VGGMQLRFAR LSEHATAPTR GSARAAGYDL YSAYDYTIPP MEKAVVKTDI QIALPSGCYG RVAPRSGLAA KHFIDVGAGV IDEDYRGNVG VVLFNFGKEK FEVKKGDRIA QLICERIFYP EIEEVQALDD TERGSGGFGS TGKN

Purity

>90%, by SDS-PAGE

Predicted Molecular Mass

21.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human dUTPase His Protein

SDS-PAGE: Recombinant Human dUTPase His Protein [NBP1-48598]

SDS-PAGE: Recombinant Human dUTPase His Protein [NBP1-48598]

SDS-Page: Recombinant Human dUTPase Protein [NBP1-48598] - 15 % SDS-PAGE (3ug)

Formulation, Preparation and Storage

NBP1-48598
Formulation 20 mM Tris-HCl buffer (pH 8.0), 10% glycerol, 1 mM DTT, 0.1 M NaCl
Preservative No Preservative
Concentration 1 mg/ml
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: dUTPase

DUT, also known as dUTP pyrophosphatase, is a ubiquitous enzyme that functions in nucleotide metabolism. This protein, in the presence of magnesium ions, is responsible for hydrolyzing dUTP to dUMP and diphosphate. This reaction is important for keeping the intracellular dUTP concentration low so that uracil does not become incorporated into DNA. Extensive incorporation of uracil into DNA can ultimately lead to cell death. This suggests that DUT is essential for cell viability, further implying that it is a potential target for anticancer therapy. Recombinant human DUT protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Alternate Names

deoxyuridine triphosphatase, dUTP pyrophosphatasedUTP nucleotidohydrolase, dUTPasedeoxyuridine 5'-triphosphate nucleotidohydrolase, mitochondrial, EC 3.6.1.23, FLJ20622

Gene Symbol

DUT

Additional dUTPase Products

Product Documents for Recombinant Human dUTPase His Protein

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Recombinant Human dUTPase His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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