Recombinant Human Dynactin Subunit 2/DCTN2/DCTN-50 His Protein

Name: Recombinant Human Dynactin Subunit 2/DCTN2/DCTN-50 His Protein
Brand: Novus Biologicals
SKU: NBP2-23010

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-23010

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Product Specifications
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Source

E. coli

Tag

His

Conjugate

Unconjugated

Applications

SDS-PAGE

View all Dynactin Subunit 2/DCTN2 Proteins and Enzymes »

Product Specifications

Description

A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-401 of Human Dynactin Subunit 2/DCTN2/DCTN-50

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSMADPKYA DLPGIARNEP DVYETSDLPE DDQAEFDAEE LTSTSVEHII VNPNAAYDKF KDKRVGTKGL DFSDRIGKTK RTGYESGEYE MLGEGLGVKE TPQQKYQRLL HEVQELTTEV EKIKTTVKES ATEEKLTPVL LAKQLAALKQ QLVASHLEKL LGPDAAINLT DPDGALAKRL LLQLEATKNS KGGSGGKTTG TPPDSSLVTY ELHSRPEQDK FSQAAKVAEL EKRLTELETA VRCDQDAQNP LSAGLQGACL METVELLQAK VSALDLAVLD QVEARLQSVL GKVNEIAKHK ASVEDADTQS KVHQLYETIQ RWSPIASTLP ELVQRLVTIK QLHEQAMQFG QLLTHLDTTQ QMIANSLKDN TTLLTQVQTT MRENLATVEG NFASIDERMK KLGK

Purity

>90%, by SDS-PAGE

Predicted Molecular Mass

46.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human Dynactin Subunit 2/DCTN2/DCTN-50 His Protein

SDS-PAGE: Recombinant Human Dynactin Subunit 2/DCTN2/DCTN-50 His Protein [NBP2-23010]

SDS-Page: DCTN-50 Protein [NBP2-23010]

Formulation, Preparation and Storage

NBP2-23010

Formulation	20 mM Tris-HCl buffer (pH8.0), 20% glycerol, 0.15 M NaCl, 1 mM DTT
Preservative	No Preservative
Concentration	0.5 mg/ml
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Dynactin Subunit 2/DCTN2

DCTN2 is a 50-kD subunit of dynactin, a macromolecular complex consisting of 10-11 subunits ranging in size from 22 to 150 kD. Dynactin binds to both microtubules and cytoplasmic dynein. It is involved in a diverse array of cellular functions, including ER-to-Golgi transport, the centripetal movement of lysosomes and endosomes, spindle formation, chromosome movement, nuclear positioning, and axonogenesis. This subunit is present in 4-5 copies per dynactin molecule. It contains three short alpha-helical coiled-coil domains that may mediate association with self or other dynactin subunits. It may interact directly with the largest subunit (p150) of dynactin and may affix p150 in place. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. Recombinant human DCTN2 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Alternate Names

DCTN2, Dynamitin, RBP50

Gene Symbol

DCTN2

Additional Dynactin Subunit 2/DCTN2 Products

Product Documents for Recombinant Human Dynactin Subunit 2/DCTN2/DCTN-50 His Protein

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COA

Product Specific Notices for Recombinant Human Dynactin Subunit 2/DCTN2/DCTN-50 His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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