Recombinant Human ECSIT His Protein

Name: Recombinant Human ECSIT His Protein
Brand: Novus Biologicals
SKU: NBP2-23017

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-23017

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Product Specifications
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Source

E. coli

Tag

His

Conjugate

Unconjugated

Applications

SDS-PAGE

View all ECSIT Proteins and Enzymes »

Product Specifications

Description

A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 19-217 of Human ECSIT

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSGTCGAAL TGTSISQVPL PKDSTGAADP PQPHIVGIQS PDQQAALARH NPARPVFVEG PFSLWLRNKC VYYHILRADL LPPEEREVEE TPEEWNLYYP MQLDLEYVRS GWDNYEFDIN EVEEGPVFAM CMAGAHDQAT MAKWIQGLQE TNPTLAQIPV VFRLAGSTRE LQTSSAGLEE PPLPEDHQEE DDNLQRQQQG QS

Purity

>90%, by SDS-PAGE

Predicted Molecular Mass

24.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human ECSIT His Protein

SDS-PAGE: Recombinant Human ECSIT His Protein [NBP2-23017]

SDS-Page: ECSIT Protein [NBP2-23017]

Formulation, Preparation and Storage

NBP2-23017

Formulation	20 mM Tris-HCl buffer (pH 8.0), 0.1 M NaCl, 10% glycerol, 1 mM DTT
Preservative	No Preservative
Concentration	1 mg/ml
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ECSIT

ECSIT is a ubiquitously expressed protein that plays an important role as an adaptor protein in the cytosolic signal transduction cascade events triggered by Toll receptor activation. It was initially identified as a cytoplasmic protein interacting specifically with TNF receptor associated factor (TRAF)-6 in the TLR pathway. Knockdown of ECSIT results in severely impaired complex I assembly and disturbed mitochondrial function. Recombinant human ECSIT protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Alternate Names

ECSIT homolog (Drosophila), evolutionarily conserved signaling intermediate in Toll pathway, mitochondrial, likely ortholog of mouse signaling intermediate in Toll pathway evolutionarilyconserved, Protein SITPEC, signaling intermediate in Toll pathway evolutionarily conserved ortholog, SITPECsignaling intermediate in Toll pathway, evolutionarily conserved

Gene Symbol

ECSIT

Additional ECSIT Products

Product Documents for Recombinant Human ECSIT His Protein

Product Datasheets

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COA

Product Specific Notices for Recombinant Human ECSIT His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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