Recombinant Human EDF1 His Protein

Name: Recombinant Human EDF1 His Protein
Brand: Novus Biologicals
SKU: NBP1-45307

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-45307

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Product Specifications
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Source

E. coli

Tag

His

Conjugate

Unconjugated

Applications

SDS-PAGE

View all EDF1 Proteins and Enzymes »

Product Specifications

Description

A recombinant protein with a C-Terminal His-tag and corresponding to the amino acids 1-148 of Human EDF1

Source: E.coli

Amino Acid Sequence: MAESDWDTVT VLRKKGPTAA QAKSKQAILA AQRRGEDVET SKKWAAGQNK QHSITKNTAK LDRETEELHH DRVTLEVGKV IQQGRQSKGL TQKDLATKIN EKPQVIADYE SGRAIPNNQV LGKIERAIGL KLRGKDIGKP IEKGPRAKLE HHHHHH

Purity

>90%, by SDS-PAGE

Predicted Molecular Mass

17.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human EDF1 His Protein

Western Blot: Recombinant Human EDF1 His Protein [NBP1-45307]

Western Blot: EDF1 Protein [NBP1-45307] - 15% SDS-PAGE (3ug)

Formulation, Preparation and Storage

NBP1-45307

Formulation	20 mM Tris-HCl buffer (pH 8.0), 1 mM DTT, 20% glycerol, 0.1 M NaCl
Preservative	No Preservative
Concentration	0.5 mg/ml
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: EDF1

Endothelial differentiation-related factor 1, also known as EDF1 may regulate endothelial cell differentiation. It has been postulated that the protein functions as a bridging molecule that interconnects regulatory proteins and the basal transcriptional machinery, thereby modulating the transcription of genes involved in endothelial differentiation. Also, EDF1 binds calmodulin thorough its IQ domain and regulates nitric oxide synthase activity through calmodulin sequestration in the cytoplasm. Though ubiquitously expressed, EDF1 is most abundant in adult liver, heart, adipose tissues, intestine and pancreas. Recombinant human EDF1 protein, fused to His-tag at C-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Alternate Names

EDF-1Multiprotein-bridging factor 1, endothelial differentiation-related factor 1, MBF1, MGC9058, multiprotein bridging factor 1, multiprotein bridging factor-1

Gene Symbol

EDF1

Additional EDF1 Products

Product Documents for Recombinant Human EDF1 His Protein

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COA

Product Specific Notices for Recombinant Human EDF1 His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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