Recombinant Human EEF1E1 His Protein

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-51088

Novus Biologicals, part of Bio-Techne
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NBP1-51088-0.1mg
NBP1-51088-0.5mg
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Key Product Details

Source

E. coli

Tag

His

Conjugate

Unconjugated

Applications

SDS-PAGE

View all EEF1E1 Proteins and Enzymes »

Product Specifications

Description

A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-174 of Human EEF1E1

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MAAAAELSLL EKSLGLSKGN KYSAQGERQI PVLQTNNGPS LTGLTTIAAH LVKQANKEYL LGSTAEEKAI VQQWLEYRVT QVDGHSSKND IHTLLKDLNS YLEDKVYLTG YNFTLADILL YYGLHRFIVD LTVQEKEKYL NVSRWFCHIQ HYPGIRQHLS SVVFIKNRLY TNSH

Purity

>95%, by SDS-PAGE

Predicted Molecular Mass

21.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human EEF1E1 His Protein

SDS-PAGE: Recombinant Human EEF1E1 His Protein [NBP1-51088]

SDS-PAGE: Recombinant Human EEF1E1 His Protein [NBP1-51088]

SDS-Page: Recombinant Human EEF1E1 Protein [NBP1-51088] - 15 % SDS-PAGE (3ug)

Formulation, Preparation and Storage

NBP1-51088
Formulation 20 mM Tris-HCl buffer (pH8.0), 20% glycerol, 0.1 M NaCl, 1 mM DTT
Preservative No Preservative
Concentration 1 mg/ml
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: EEF1E1

EEF1E1, also known as, eukaryotic translation elongation factor 1 epsilon-1, shares sequence similarity with the amino-terminal ends of the Beta and Gamma subunits of EF-1. By specifically interacting with MetRS, this protein binds to a macromolecular tRNA synthtase complex that catalyzes the ligation of specific amino acids to their cognate tRNAs. Upon DNA damage, it translocates to the nucleus where it interacts with ATM and ATR, resulting in p53 activation. Recombinant human EEIF1E1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.

Alternate Names

AIMP3Aminoacyl tRNA synthetase complex-interacting multifunctional protein 3, ARS-interacting multifunctional protein 3, Elongation factor p18, eukaryotic translation elongation factor 1 epsilon 1, eukaryotic translation elongation factor 1 epsilon-1, Multisynthase complex auxiliary component p18, P18, p18 component of aminoacyl-tRNA synthetase complex

Gene Symbol

EEF1E1

Additional EEF1E1 Products

Product Documents for Recombinant Human EEF1E1 His Protein

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Recombinant Human EEF1E1 His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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