Recombinant Human EGF Protein

Name: Recombinant Human EGF Protein
Brand: Novus Biologicals
SKU: NBP2-34952
Rating: 5 (1 reviews)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-34952

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Citations (16)

Product Datasheet / COA

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Product Specifications
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Source

E. coli

Accession #

P01133

Conjugate

Unconjugated

Applications

Bioactivity, SDS-PAGE

View all EGF Proteins and Enzymes »

Product Specifications

Description

A single non-glycosylated polypeptide chain containing 53 amino acids corresponding to EGF Source: E. coli

Uniprot ID: P01133

Amino Acid Sequence: NSDSECPLSH DGYCLHDGVC MYIEALDKYA CNCVVGYIGE RCQYRDLKWW ELR

Purity

>95%, by SDS-PAGE and HPLC

Endotoxin Level

Less than 1 EU/ug of EGF as determined by LAL method.

Predicted Molecular Mass

6.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine Balb/c 3T3 cells is less than 1 ng/ml, corresponding to a specific activity of > 1.0 x 10^6 IU/mg.

Protein / Peptide Type

Recombinant Protein

Reviewed Applications

Read 1 review rated 5 using NBP2-34952 in the following applications:

SDS-PAGE Control (1 Review)

Scientific Data Images for Recombinant Human EGF Protein

SDS-PAGE: Recombinant Human EGF Protein [NBP2-34952]

SDS-Page: Human EGF Protein [NBP2-34952]

Formulation, Preparation and Storage

NBP2-34952

Formulation	Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.4.
Preservative	No Preservative
Concentration	Lyoph
Reconstitution	Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL.
Format	Carrier-Free
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.

Background: EGF

EGF R (ErbB1) is a receptor tyrosine kinase that is widely expressed on epithelial cells and is required for epithelial cell development and proliferation. EGF R binds the EGF family ligands: EGF, Amphiregulin, TGF-alpha, Betacellulin, Epiregulin, HB-EGF, and Epigen. Ligand binding induces EGF R homodimerization as well as heterodimerization with ErbB2, ErbB3, or ErbB4. EGF R signaling regulates multiple biological functions including cell proliferation, differentiation, motility, and apoptosis. Alterations in the structure, expression, and signaling of EGF R are involved in the development and metastasis of a wide variety of cancers.

Long Name

Epidermal Growth Factor

Alternate Names

HOMG4, URG, Urogastrone

Gene Symbol

EGF

UniProt