Recombinant Rat EGF Protein
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-35293
Key Product Details
Product Specifications
Description
A single non-glycosylated polypeptide chain containing 53 amino acids corresponding to EGF, including 3 intramolecular disulfide-bonds Source: E. coli
Uniprot ID: P07522
Amino Acid Sequence: NSNTGCPPSY DGYCLNGGVC MYVESVDRYV CNCVIGYIGE RCQHRDLRWW KLR
Purity
> 97 % pure by SDS-PAGE and HPLC
Endotoxin Level
Less than 1 EU/ug of EGF as determined by LAL method.
Predicted Molecular Mass
6.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Activity
EGF protein is fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine Balb/c 3T3 cells is less than 0.1 ng/ml, corresponding to a specific activity of > 1.0 x 10^7 IU/mg
Protein / Peptide Type
Recombinant Protein
Scientific Data Images for Recombinant Rat EGF Protein
![SDS-PAGE: Recombinant Rat EGF Protein [NBP2-35293]](https://resources.bio-techne.com/images/products/Rat-EGF-Protein-SDS-Page-NBP2-35293-img0001.jpg "SDS-PAGE: Recombinant Rat EGF Protein [NBP2-35293]")
SDS-PAGE: Recombinant Rat EGF Protein [NBP2-35293]
SDS-Page: Rat EGF Protein [NBP2-35293]Formulation, Preparation and Storage
NBP2-35293
| Formulation | Lyophilized from a 0.2 um filtered solution in PBS, pH 7.4. |
| Preservative | No Preservative |
| Concentration | Lyoph |
| Reconstitution | Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL. Apportion stock solutions into working aliquots and store at |
| Format | Carrier-Free |
| Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles. |
Background: EGF
Long Name
Epidermal Growth Factor
Alternate Names
HOMG4, URG, Urogastrone
Gene Symbol
EGF
UniProt
Additional EGF Products
Product Documents for Recombinant Rat EGF Protein
Product Specific Notices for Recombinant Rat EGF Protein
This lyophilized preparation is stable at 2-8 degrees C, but should be kept at -20 degrees C for long term storage, preferably desiccated. Upon reconstitution, the preparation is most stable at -20 to -80 degrees C, and can be stored for one week at 2-8 degrees C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 degrees C to -80 degrees C. Avoid repeated freeze/thaw cycles.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.
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