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Recombinant Human eIF5A2 His Protein

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-45261

Novus Biologicals, part of Bio-Techne
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NBP1-45261-0.05mg
NBP1-45261-0.25mg

Key Product Details

Source

E. coli

Tag

His

Conjugate

Unconjugated

Applications

SDS-PAGE

Product Specifications

Description

A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-153 of Human eIF5A2

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MADEIDFTTG DAGASSTYPM QCSALRKNGF VVLKGRPCKI VEMSTSKTGK HGHAKVHLVG IDIFTGKKYE DICPSTHNMD VPNIKRNDYQ LICIQDGYLS LLTETGEVRE DLKLPEGELG KEIEGKYNAG EDVQVSVMCA MSEEYAVAIK PCK

Purity

>85%, by SDS-PAGE

Predicted Molecular Mass

18.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human eIF5A2 His Protein

Western Blot: Recombinant Human eIF5A2 His Protein [NBP1-45261]

Western Blot: Recombinant Human eIF5A2 His Protein [NBP1-45261]

Western Blot: eIF5A2 Protein [NBP1-45261] - 15% SDS-PAGE (3ug)

Formulation, Preparation and Storage

NBP1-45261
Formulation 20 mM Tris-HCl buffer (pH 8.0), 10% glycerol
Preservative No Preservative
Concentration 0.5 mg/ml
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: eIF5A2

EIF5A2, a member of eukaryotic initiation factor 5A subfamily, is an essential protein tightly linked to cellular polyamine homeostasis. The precise role of eIF-5A in protein biosynthesis is not known but it functions by promoting the formation of the first peptide bond during the initial stage of protein synthesis. It seems to be the only eukaryotic protein to have a hypusine residue, which is a post-translational modification of a lysine by the addition of a butylamino group. Recombinant human EIF5A2 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.

Alternate Names

EIF-5A2, eIF-5A-2, eIF5AII, eukaryotic initiation factor 5A, Eukaryotic initiation factor 5A isoform 2, eukaryotic translation initiation factor 5A2, eukaryotic translation initiation factor 5A-2

Gene Symbol

EIF5A2

Additional eIF5A2 Products

Product Documents for Recombinant Human eIF5A2 His Protein

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Recombinant Human eIF5A2 His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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