Recombinant Human EMAP-II/AIMP1 Protein

Name: Recombinant Human EMAP-II/AIMP1 Protein
Brand: Novus Biologicals
SKU: NBP2-34954

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-34954

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Product Specifications
Scientific Data
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Key Product Details

Source

E. coli

Accession #

Q12904

Conjugate

Unconjugated

Applications

Bioactivity, SDS-PAGE

View all EMAP-II Proteins and Enzymes »

Product Specifications

Description

A single non-glycosylated polypeptide chain containing 166 amino acids corresponding to EMAP-II/AIMP1 Source: E. coli

Uniprot ID: Q12904

Amino Acid Sequence: SKPIDVSRLD LRIGCIITAR KHPDADSLYV EEVDVGEIAP RTVVSGLVNH VPLEQMQNRM VILLCNLKPA KMRGVLSQAM VMCASSPEKI EILAPPNGSV PGDRITFDAF PGEPDKELNP KKKIWEQIQP DLHTNDECVA TYKGVPFEVK GKGVCRAQTM SNSGIK

This lyophilized preparation is stable for 12 months from date of receipt at -20 to -70 degrees C, preferably desiccated. Upon reconstitution, the preparation can be stored for 1 month at 2-8 degrees C under sterile conditions, and for 3 months at -20 degrees C to -70 degrees C. .Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Stock solutions should be aliquoted and stored at < -20 degrees C. Further dilutions should be made in appropriate buffered solutions

Purity

> 98 % pure by SDS-PAGE and HPLC

Endotoxin Level

Less than 1 EU/ug of EMAP-II/AIMP1 as determined by LAL method.

Predicted Molecular Mass

18.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

Fully biologically active when compared to standard. The ED50 as determined by the apoptotic effect using serum free human MCF-7 cells is less than 40 ng/ml, corresponding to a specific activity of > 2.5 x 10^4 IU/mg.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human EMAP-II/AIMP1 Protein

SDS-PAGE: Recombinant Human EMAP-II/AIMP1 Protein [NBP2-34954]

SDS-Page: Recombinant Human EMAP-II/AIMP1 Protein [NBP2-34954] - SDS-PAGE of NBP2-34954, showing a band at approximately 18 kDa.

Formulation, Preparation and Storage

NBP2-34954

Formulation	Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.4.
Preservative	No Preservative
Concentration	Lyoph
Reconstitution	Recommended to be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL
Format	Carrier-Free
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.

Background: EMAP-II

EMAP-II is a tumor derived cytokine that exerts a wide range of activities on endothelial cells, monocytes and neutrophils. EMAP-II inhibits endothelial cell proliferation, vasculogenesis, neovessel formation, and can induce apoptosis. It is also chemotactic towards neutrophils and monocytes and induces myeloperoxidase activity from neutrophils. Of clinical importance, EML2 (EMAP-II) inhibits angiogenesis of vascular beds and suppresses the growth of primary and secondary tumors without affecting normal tissues. Mature EMAP-II is an 18.3 kDa protein, which is synthesized as the C-terminal portion of a biologically inactive precursor protein containing a propeptide of 146 amino acid residues.

Long Name

Endothelial-Monocyte Activating Polypeptide II

Alternate Names

AIMP1, EMAPII, SCYE1

Gene Symbol

AIMP1

UniProt