Recombinant Human EMP/MAEA His Protein

Name: Recombinant Human EMP/MAEA His Protein
Brand: Novus Biologicals
SKU: NBP2-23208

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-23208

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Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Source

E. coli

Tag

His

Conjugate

Unconjugated

Applications

SDS-PAGE

View all EMP/MAEA Proteins and Enzymes »

Product Specifications

Description

A denatured recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-396 of Human EMP/MAEA

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSMAVQESA AQLSMTLKVQ EYPTLKVPYE TLNKRFRAAQ KNIDRETSHV TMVVAELEKT LSGCPAVDSV VSLLDGVVEK LSVLKRKAVE SIQAEDESAK LCKRRIEHLK EHSSDQPAAA SVWKRKRMDR MMVEHLLRCG YYNTAVKLAR QSGIEDLVNI EMFLTAKEVE ESLERRETAT CLAWCHDNKS RLRKMKSCLE FSLRIQEFIE LIRQNKRLDA VRHARKHFSQ AEGSQLDEVR QAMGMLAFPP DTHISPYKDL LDPARWRMLI QQFRYDNYRL HQLGNNSVFT LTLQAGLSAI KTPQCYKEDG SSKSPDCPVC SRSLNKLAQP LPMAHCANSR LVCKISGDVM NENNPPMMLP NGYVYGYNSL LSIRQDDKVV CPRTKEVFHF SQAEKVYIM

Purity

>85%, by SDS-PAGE

Predicted Molecular Mass

47.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Application Notes

Denatured protein is most likely not the best option for functional studies. It is better suited for Western Blot (WB) or imaging assays.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human EMP/MAEA His Protein

SDS-PAGE: Recombinant Human EMP/MAEA His Protein [NBP2-23208]

SDS-Page: Recombinant Human EMP/MAEA Protein [NBP2-23208]

Formulation, Preparation and Storage

NBP2-23208

Formulation	20 mM Tris-HCl buffer (pH 8.0), 1 M UREA, 10% glycerol
Preservative	No Preservative
Concentration	0.25 mg/ml
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: EMP/MAEA

Macrophage erythroblast attacher isoform 1, also known as MAEA, is a 396 amino acid ubiquitously expressed adhesion protein. Expressed as 5 alternatively spliced isoforms, MAEA contains one CTLH domain and one LisH domain. MAEA can form a complex with F-actin, which is involved regulating actin distribution in erythroblasts and macrophages. Considered to assist with cell division and nuclear architecture, MAEA is localized with condensed chromatin at prophase, nuclear spindle poles at metaphase and in the contractile ring during telophase and cytokinesis. Recombinant human MAEA protein, fused to His-tag at N-terminus, was expressed in E.coli.

Long Name

Erythroblast Macrophage Protein/Macrophage Erythroblast Attacher

Alternate Names

EMLP, HLC-10, MAEA, PIG5

Gene Symbol

MAEA

Additional EMP/MAEA Products

Product Documents for Recombinant Human EMP/MAEA His Protein

Product Datasheets

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COA

Product Specific Notices for Recombinant Human EMP/MAEA His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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