Recombinant Human EPGN Protein

Name: Recombinant Human EPGN Protein
Brand: Novus Biologicals
SKU: NBP2-34987

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-34987

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Product Datasheet / COA

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Product Specifications
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Source

E. coli

Accession #

Q6UW88

Conjugate

Unconjugated

Applications

Bioactivity, SDS-PAGE

View all EPGN Proteins and Enzymes »

Product Specifications

Description

A monomeric protein containing 72 amino acid residues, which comprises the EGF homologous portion of the Epigen precursor Source: E. coli

Uniprot ID: Q6UW88

Amino Acid Sequence: AVTVTPPITA QQADNIEGPI ALKFSHLCLE DHNSYCINGA CAFHHELEKA ICRCFTGYTG ERCEHLTLTS YA

This lyophilized preparation is stable for 12 months from date of receipt at -20 to -70 degrees C, preferably desiccated. Upon reconstitution, the preparation can be stored for 1 month at 2-8 degrees C under sterile conditions, and for 3 months at -20 degrees C to -70 degrees C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Stock solutions should be aliquoted and stored at < -20 degrees C. Further dilutions should be made in appropriate buffered solutions

Purity

> 98 % pure by SDS-PAGE and HPLC

Endotoxin Level

Less than 1 EU/ug of EPGN as determined by LAL method.

Predicted Molecular Mass

7.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

EPGN protein is fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine Balb/c 3T3 cells is less than 300 ng/ml, corresponding to a specific activity of > 3.3 x 10^3 IU/mg.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human EPGN Protein

SDS-PAGE: Recombinant Human EPGN Protein [NBP2-34987]

SDS-Page: Human EPGN Protein [NBP2-34987]

Formulation, Preparation and Storage

NBP2-34987

Formulation	Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.4.
Preservative	No Preservative
Concentration	Lyoph
Reconstitution	Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL. Apportion stock solutions into working aliquots and store at
Format	Carrier-Free
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.

Background: EPGN

Epigen is a member of the epidermal growth factor family and is expressed by several tissues, including the testis, liver, heart and in certain tumor cells. It is strongly mitogenic for fibroblasts and epithelial cells, despite its relatively weak affinity for its main receptor, ErbB1. The mitogenic potential of Epigen is enhanced by its unusually long persistence on the membrane before ubiquitylation and receptor-mediated depletion. The gene of human Epigen encodes a 154 amino acid (a.a.) residue type I transmembrane precursor glycoprotein which contains a 22 a.a. signal peptide, an 88 a.a. extracellular domain, a 21 a.a. transmembrane domain and a 23 a.a. cytoplasmic domain. By alternative splicing of the gene, Epigen has several isoforms. The mature, shed form of human Epigen (54 - 104a.a.) shares 92 %, 94 % and 94 % a.a. sequence identity with mouse, rat and equine Epigen, respectively.

Alternate Names

epigen, epithelial mitogen homolog (mouse), PRO9904, UNQ3072/PRO9904

Gene Symbol

EPGN

UniProt