What is the sequence of the His-Tag? Is it N-terminal or C-terminal? I am using the protein in mass spectrometry experiments and knowing the exact sequence is very important.

The amino acid sequence of the His-tag is: MGSSHHHHHH SSGLVPRGSH The His-tag is N-terminal.

Do you have any info on the potential activity or 3D structure of product NBP1-30309? Do you expect this protein to be folded into the correct tertiary structure? Any info on tag (6x his) effects for this protein? Do you have any information from a CD spectra or solubility?

NBP1-30309 comes in 20 mM Tris-HCl buffer (pH8.0) containing 1mM DTT and 10% glycerol. In this reducing buffer, it is not expected to have proper folding and/or activity. This protein is intended to be used as a positive control for Western blots and ELISA.

Recombinant Human ERK2 His Protein

Name: Recombinant Human ERK2 His Protein
Brand: Novus Biologicals
SKU: NBP1-30309

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-30309

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Product Datasheet / COA

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Product Specifications
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Source

E. coli

Tag

His

Conjugate

Unconjugated

Applications

SDS-PAGE

View all ERK2 Proteins and Enzymes »

Product Specifications

Description

A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-360 of Human ERK2

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MAAAAAAGAG PEMVRGQVFD VGPRYTNLSY IGEGAYGMVC SAYDNVNKVR VAIKKISPFE HQTYCQRTLR EIKILLRFRH ENIIGINDII RAPTIEQMKD VYIVQDLMET DLYKLLKTQH LSNDHICYFL YQILRGLKYI HSANVLHRDL KPSNLLLNTT CDLKICDFGL ARVADPDHDH TGFLTEYVAT RWYRAPEIML NSKGYTKSID IWSVGCILAE MLSNRPIFPG KHYLDQLNHI LGILGSPSQE DLNCIINLKA RNYLLSLPHK NKVPWNRLFP NADSKALDLL DKMLTFNPHK RIEVEQALAH PYLEQYYDPS DEPIAEAPFK FDMELDDLPK EKLKELIFEE TARFQPGYRS

Purity

>95%, by SDS-PAGE

Predicted Molecular Mass

43.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human ERK2 His Protein

SDS-PAGE: Recombinant Human ERK2 His Protein [NBP1-30309]

SDS-Page: Recombinant Human ERK2 Protein [NBP1-30309] - 15% SDS-Page (3ug)

Formulation, Preparation and Storage

NBP1-30309

Formulation	20 mM Tris-HCl buffer (pH 8.0), 10% glycerol, 1 mM DTT, 0.1 M NaCl
Preservative	No Preservative
Concentration	1 mg/ml
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ERK2

MAPK1, also known as ERK(extracellular signal-regulated kinase), acts as an integration point for multiple biochemical signals, and is involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. The activation of this kinase requires its phosphorylation by upstream kinases. Upon activation, this kinase translocates to the nucleus of the stimulated cells, where it phosphorylates nuclear targets. Recombinant human MAPK1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Long Name

Extracellular Signal-regulated Kinase 2

Alternate Names

ERT1, MAPK1, MAPK2, p41mapk, p42mapk, PRKM1, PRKM2

Gene Symbol

MAPK1

Additional ERK2 Products

Product Documents for Recombinant Human ERK2 His Protein

Product Datasheets

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COA

Product Specific Notices for Recombinant Human ERK2 His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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