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FAM105B/OTULIN Recombinant Protein Antigen

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-14722PEP

Novus Biologicals, part of Bio-Techne
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NBP2-14722PEP

Key Product Details

Source

E. coli

Conjugate

Unconjugated

Applications

Antibody Competition

Product Specifications

Description

A recombinant protein antigen with a N-terminal His6-ABP tag corresponding to human FAM105B.

Source: E. coli

Amino Acid Sequence: DEIEKEKELLIHERGASEPRLSVAPEMDIMDYCKKEWRGNTQKATCMKMGYEEVSQKFTSIRRVRGDNYCALRATLFQAMSQAVGLPPWLQDPELMLLP

Fusion Tag: N-terminal His6ABP (ABP = Albumin Binding Protein derived from Streptococcal Protein G)

This product is intended to be used as a blocking antigen for antibody competition assays. Any other use of this antigen is done at the risk of the user. The use of this product for commercial production is strictly prohibited. Please contact technical support if you have any questions.

Purity

>80% by SDS-PAGE and Coomassie blue staining

Predicted Molecular Mass

29 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Applications

Antibody Competition (10 - 100 molar excess)

Application Notes

This recombinant antigen is only intended to be used as a blocking agent to confirm antibody specificity with the corresponding antibody, catalog number NBP2-14722.

It is purified by IMAC chromatography, and the expected concentration is greater than 0.5 mg/ml.

For current lot information, including availability, please contact our technical support team click nb-technical@bio-techne.com

Protein / Peptide Type

Recombinant Protein Antigen

Formulation, Preparation and Storage

NBP2-14722PEP
Formulation PBS and 1M Urea, pH 7.4.
Preservative No Preservative
Concentration Please see the vial label for concentration. If unlisted please contact technical services.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at -20C. Avoid freeze-thaw cycles.

Background: FAM105B/OTULIN

Family with Sequence Similarity 105, Member B (FAM105B), also known as OTULIN, is a 352 amino acid (aa) member of the ovarian tumor protein superfamily with a predicted molecular weight of 40 kDa. The mouse FAM105B/OTULIN ortholog shares 89% aa sequence identity with the human protein. It is a C65 type peptidase that has high specificity for methionine-1-linked linear Ubiquitin chains and contributes to the regulation of NFkB signaling.

Long Name

Family with Sequence Similarity 105, Member B

Alternate Names

OTULIN

Gene Symbol

OTULIN

Additional FAM105B/OTULIN Products

Product Documents for FAM105B/OTULIN Recombinant Protein Antigen

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for FAM105B/OTULIN Recombinant Protein Antigen

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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