We are interested in ordering Novus' Fascin recombinant protein, catalog number: NBP1-45313 provided at 1.0 mg/mL. Has any testing been performed on the Fascin protein studying formation of the bundling of actin filaments by visualizing the actin bundles under a fluorescent microscope?

Name: Recombinant Human Fascin His Protein
Brand: Novus Biologicals
SKU: NBP1-45313

Thus far Novus Biologicals has only validated our protein for use in a SDS-PAGE application. We have not yet performed Immunofluorescence to visualize whether the Fascin protein forms the bundling of actin filaments. If you are willing to test the recombinant protein for visualization of this process we would encourage you to apply for our Innovators Reward Program. Under the terms of this program, you will be eligible for a full credit in exchange for your data, in the form of an online review, regardless of positive or negative results.

Recombinant Human Fascin His Protein

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-45313

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Product Datasheet / COA

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Product Specifications
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Source

E. coli

Tag

His

Conjugate

Unconjugated

Applications

SDS-PAGE

View all Fascin Proteins and Enzymes »

Product Specifications

Description

A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-493 of Human Fascin

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MTANGTAEAV QIQFGLINCG NKYLTAEAFG FKVNASASSL KKKQIWTLEQ PPDEAGSAAV CLRSHLGRYL AADKDGNVTC EREVPGPDCR FLIVAHDDGR WSLQSEAHRR YFGGTEDRLS CFAQTVSPAE KWSVHIAMHP QVNIYSVTRK RYAHLSARPA DEIAVDRDVP WGVDSLITLA FQDQRYSVQT ADHRFLRHDG RLVARPEPAT GYTLEFRSGK VAFRDCEGRY LAPSGPSGTL KAGKATKVGK DELFALEQSC AQVVLQAANE RNVSTRQGMD LSANQDEETD QETFQLEIDR DTKKCAFRTH TGKYWTLTAT GGVQSTASSK NASCYFDIEW RDRRITLRAS NGKFVTSKKN GQLAASVETA GDSELFLMKL INRPIIVFRG EHGFIGCRKV TGTLDANRSS YDVFQLEFND GAYNIKDSTG KYWTVGSDSA VTSSGDTPVD FFFEFCDYNK VAIKVGGRYL KGDHAGVLKA SAETVDPASL WEY

Purity

>95%, by SDS-PAGE

Predicted Molecular Mass

56.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human Fascin His Protein

SDS-PAGE: Recombinant Human Fascin His Protein [NBP1-45313]

SDS-Page: Recombinant Human Fascin Protein [NBP1-45313] - 15% SDS-PAGE (3ug)

Formulation, Preparation and Storage

NBP1-45313

Formulation	20 mM Tris-HCl buffer (pH8.0), 20% glycerol, 2 mM DTT, 100 mM NaCl
Preservative	No Preservative
Concentration	1 mg/ml
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Fascin

FSCN1 is an actin-bundling protein that provides rigidity to filopodial bundles to efficiently push the membrane forward during cytoskeleton remodeling and cell migration. Probably involved in the assembly of actin filament bundles present in microspikes, membrane ruffles, and stress fibers. And this protein is absent from most normal epithelia yet is upregulated in multiple forms of human carcinoma, where its expression correlates clinically with a poor prognosis. FSCN1 expression has been localized to neurons, glial cells, and endothelial cells. Recombinant human FSCN1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Alternate Names

FAN1, FSCN1, HSN, Singed-like Protein, SNL

Gene Symbol

FSCN1

Additional Fascin Products

Product Documents for Recombinant Human Fascin His Protein

Product Datasheets

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COA

Product Specific Notices for Recombinant Human Fascin His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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