Recombinant Rat FGF-10 Protein

Name: Recombinant Rat FGF-10 Protein
Brand: Novus Biologicals
SKU: NBP2-35261

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-35261

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Product Specifications
Scientific Data
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Background
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Key Product Details

Source

E. coli

Accession #

P70492

Conjugate

Unconjugated

Applications

Bioactivity, SDS-PAGE

View all FGF-10 Proteins and Enzymes »

Product Specifications

Description

A single non-glycosylated polypeptide chain containing 179 amino acids corresponding to FGF-10 Source: E. coli

Uniprot ID: P70492

Amino Acid Sequence: QALGQDMVSP EATNSSSSSS SSSSSSSFSS PSSAGRHVRS YNHLQGDVRW RKLFSFTKYF LKIEKNGKVS GTKKENCPYS ILEITSVEIG VVAVKAINSN YYLAMNKKGK LYGSKEFNND CKLKERIEEN GYNTYASFNW QHNGRQMYVA LNGKGAPRRG QKTRRKNTSA HFLPMVVHS

This lyophilized preparation is stable at 2-8 degrees C, but should be kept at -20 degrees C for long term storage, preferably desiccated. Upon reconstitution, the preparation is most stable at -20 to -80 degrees C, and can be stored for one week at 2-8 degrees C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 degrees C to -80 degrees C. Avoid repeated freeze/thaw cycles.

Purity

> 97 % pure by SDS-PAGE and HPLC

Endotoxin Level

Less than 1 EU/ug of FGF-10 as determined by LAL method.

Predicted Molecular Mass

20 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

FGF10 Protein is fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using monkey 4MBr-5 cells is less than 120 ng/ml, corresponding to a specific activity of > 8.3 x 10^3 IU/mg.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Rat FGF-10 Protein

SDS-PAGE: Recombinant Rat FGF-10 Protein [NBP2-35261]

SDS-Page: Rat FGF-10 Protein [NBP2-35261]

Formulation, Preparation and Storage

NBP2-35261

Formulation	Lyophilized from a 0.2 um filtered concentrated solution in 20 mM Tris, 500 mM NaCl, pH 7.4.
Preservative	5% Trehalose
Concentration	Lyoph
Reconstitution	Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL. Apportion stock solutions into working aliquots and store at
Format	Carrier-Free
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.

Background: FGF-10

FGF-10 was originally identified from rat embryos by homology-based polymerase chain reaction. Rat FGF-10 shares approximately 95 % amino acid sequence identity with human FGF-10. Among the FGF family members, FGF-10 is most closely related to FGF-7. The expression of FGF-10 transcripts has been shown to be most abundant in the embryo and adult lung. Recombinant FGF-10 preparations have been shown to be mitogenic for epithelial and epidermal cells but not fibroblasts. Based on its in vitro biological activities and in vivo expression pattern, FGF-10 has been proposed to play unique roles in the brain, in lung development, wound healing and limb bud formation.

Long Name

Fibroblast Growth Factor 10

Alternate Names

FGF10, KGF-2, KGF2, KGF-2/FGF-10

Gene Symbol

FGF10

UniProt