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Recombinant Human FGF-12 Protein

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-61375

Novus Biologicals, part of Bio-Techne
Catalog #
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NBP2-61375-100ug
NBP2-61375-10ug
NBP2-61375-1mg
NBP2-61375-500ug

Key Product Details

Source

E. coli

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Description

A single non-glycosylated polypeptide chain containing 181 amino acids corresponding to FGF-12 Source: E. coli

Amino Acid Sequence: MESKEPQLKG IVTRLFSQQG YFLQMHPDGT IDGTKDENSD YTLFNLIPVG LRVVAIQGVK ASLYVAMNGE GYLYSSDVFT PECKFKESVF ENYYVIYSST LYRQQESGRA WFLGLNKEGQ IMKGNRVKKT KPSSHFVPKP IEVCMYREQS LHEIGEKQGR SRKSSGTPTM NGGKVVNQDS T

Purity

>98%, by SDS-PAGE and HPLC

Endotoxin Level

Less than 0.1 EU/ug of FGF-12 as determined by LAL method.

Predicted Molecular Mass

20.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

The biological activity was determined by its binding ability in a functional ELISA. Immobilized rHuFGF R4/Fc Chimera at 5 ug/mL (100 uL/well) can bind rHuFGF-12 with a linear range of 1.6-100 ng/mL.

Protein / Peptide Type

Recombinant Protein

Formulation, Preparation and Storage

NBP2-61375
Formulation Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH7.4, with 1 mM DTT.
Preservative No Preservative
Concentration Lyoph
Reconstitution Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL. Apportion stock solutions into working aliquots and store at
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.

Background: FGF-12

FGF-12 is a member of the FGF superfamily of heparin-binding mitogenic molecules characterized by the presence of a core, 120 amino acid (aa) beta-trefoil structure. FGF-11, -12, -13, -14, originally termed FGF homologous factors (FHF) -3, -1, -2, and -4, respectively, form a subgroup within the FGF family. Human FGF-12/FHF-1 is synthesized as a 243 aa protein. It lacks a typical signal sequence and is considered cytoplasmic. It also possess an N-terminal bipartite nuclear localization signal at aa 11-18 and 28-38.

Long Name

Fibroblast Growth Factor 12

Alternate Names

FGF12, FHF1

Gene Symbol

FGF12

Additional FGF-12 Products

Product Documents for Recombinant Human FGF-12 Protein

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Recombinant Human FGF-12 Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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