Recombinant Rat FGF-9 Protein

Name: Recombinant Rat FGF-9 Protein
Brand: Novus Biologicals
SKU: NBP2-35196

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-35196

Submit a review

Product Datasheet / COA

Catalog #

Availability

Size / Price

Qty

100% Guarantee

Product Specifications
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Source

E. coli

Accession #

P36364

Conjugate

Unconjugated

Applications

Bioactivity, SDS-PAGE

View all FGF-9 Proteins and Enzymes »

Product Specifications

Description

A single non-glycosylated polypeptide chain containing 205 amino acids corresponding to FGF-9 Source: E. coli

Uniprot ID: P36364

Amino Acid Sequence: LGEVGSYFGV QDAVPFGNVP VLPVDSPVLL SDHLGQSEAG GLPRGPAVTD LDHLKGILRR RQLYCRTGFH LEIFPNGTIQ GTRKDHSRFG ILEFISIAVG LVSIRGVDSG LYLGMNEKGE LYGSEKLTQE CVFREQFEEN WYNTYSSNLY KHVDTGRRYY VALNKDGTPR EGTRTKRHQK FTHFLPRPVD PDKVPELYKD ILSQS

Purity

>95%, by SDS-PAGE and HPLC

Endotoxin Level

Less than 1 EU/ug of FGF-9 as determined by LAL method.

Predicted Molecular Mass

23.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

FGF9 Protein is fully biologically active when compared to standard. The ED50 as determined by thymidine uptake assay using FGF-receptors transfected BaF3 cells is less than 0.5 ng/ml, corresponding to a specific activity of > 2.0 x 10^6 IU/mg.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Rat FGF-9 Protein

SDS-PAGE: Recombinant Rat FGF-9 Protein [NBP2-35196]

SDS-Page: Rat FGF-9 Protein [NBP2-35196]

Formulation, Preparation and Storage

NBP2-35196

Formulation	Lyophilized from a 0.2 um filtered concentrated solution in 20 mM Tris, 400 mM NaCl, pH 8.0.
Preservative	No Preservative
Concentration	Lyoph
Reconstitution	Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL.
Format	Carrier-Free
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.

Background: FGF-9

Fibroblast growth factor-9 (FGF-9) is a member of the fibroblast growth factor (FGF) family. All FGF family members are heparin binding growth factors with a core 120 amino acid (a.a.) FGF domain that allows for a common tertiary structure. FGF-9 plays an important role in the regulation of embryonic development, cell proliferation, cell differentiation and cell migration. This protein was isolated as a secreted factor that exhibits a growth-stimulating effect on cultured glial cells. FGF-9 is a monomer and interacts with FGFR1, FGFR2, FGFR3 and FGFR4. The rat FGF-9 shares 98 % a.a. sequence identity with human FGF-9.

Long Name

Fibroblast Growth Factor 9

Alternate Names

FGF9

Gene Symbol