Recombinant Rat FGF18 Protein

Name: Recombinant Rat FGF18 Protein
Brand: Novus Biologicals
SKU: NBP2-35296

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-35296

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Product Specifications
Scientific Data
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Background
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Key Product Details

Source

E. coli

Conjugate

Unconjugated

Applications

Bioactivity, SDS-PAGE

View all FGF-18 Proteins and Enzymes »

Product Specifications

Description

A single non-glycosylated polypeptide chain containing 180 amino acids corresponding to FGF18 Source: E. coli

Uniprot ID: O88182

Amino Acid Sequence: EENVDFRIHV ENQTRARDDV SRKQLRLYQL YSRTSGKHIQ VLGRRISARG EDGDKYAQLL VETDTFGSQV RIKGKETEFY LCMNRKGKLV GKPDGTSKEC VFIEKVLENN YTALMSAKYS GWYVGFTKKG RPRKGPKTRE NQQDVHFMKR YPKGQTELQK PFKYTTVTKR SRRIRPTHPG

This lyophilized preparation is stable at 2-8 degrees C, but should be kept at -20 degrees C for long term storage, preferably desiccated. Upon reconstitution, the preparation is most stable at -20 to -80 degrees C, and can be stored for one week at 2-8 degrees C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 degrees C to -80 degrees C. Avoid repeated freeze/thaw cycles.

Purity

>95%, by SDS-PAGE and HPLC

Endotoxin Level

Less than 1 EU/ug of FGF18 as determined by LAL method.

Predicted Molecular Mass

21 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

FGF18 Protein is fully biologically active when compared to standard. The ED50 as determined by thymidine uptake assay using FGF-receptors transfected BaF3 cells is less than 0.5 ng/ml, corresponding to a specific activity of > 2.0 x 10^6 IU/mg.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Rat FGF18 Protein

SDS-PAGE: Recombinant Rat FGF18 Protein [NBP2-35296]

SDS-Page: Rat FGF18 Protein [NBP2-35296]

Formulation, Preparation and Storage

NBP2-35296

Formulation	Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.4, 500 mM NaCl.
Preservative	No Preservative
Concentration	Lyoph
Reconstitution	Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL. Apportion stock solutions into working aliquots and store at
Format	Carrier-Free
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.

Background: FGF-18

Rat FGF-18 is encoded by the FGF18 gene in rats. By phylogenetic analysis and gene location analysis, FGF-18 is divided into FGF-8 subfamily which has three members FGF-8, FGF-17 and FGF-18. Using FGF knockout mice model, the numbers of this subfamily were testified that have crucial roles of in embryo development. FGF-18-/- mice have decreased expression of osteogenic markers and delayed long-bone ossification. FGF-18 also has significant roles in lung development and has an anabolic effect on cartilage formation. Additionally, it has been shown in vitro that this protein is able to induce neurite outgrowth in PC12 cells. Rat FGF-18 shares 98% amino acid sequence identity with human.

Long Name

Fibroblast Growth Factor 18

Alternate Names

FGF18

Gene Symbol