Recombinant Human G-CSF Protein
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-34884
Key Product Details
Product Specifications
Description
A single non-glycosylated polypeptide chain containing 174 amino acids corresponding to G-CSF Source: E. coli
Uniprot ID: P09919
Amino Acid Sequence: TPLGPASSLP QSFLLKCLEQ VRKIQGDGAA LQEKLCATYK LCHPEELVLL GHSLGIPWAP LSSCPSQALQ LAGCLSQLHS GLFLYQGLLQ ALEGISPELG PTLDTLQLDV ADFATTIWQQ MEELGMAPAL QPTQGAMPAF ASAFQRRAGG VLVASHLQSF LEVSYRVLRH LAQP
Purity
> 98 % pure by SDS-PAGE and HPLC
Endotoxin Level
Less than 1 EU/ug of G-CSF as determined by LAL method.
Predicted Molecular Mass
18.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Activity
Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine NFS-60 cells is less than 0.1 ng/ml, corresponding to a specific activity of > 1.0 x 10^7 IU/mg.
Protein / Peptide Type
Recombinant Protein
Scientific Data Images for Recombinant Human G-CSF Protein
![SDS-PAGE: Recombinant Human G-CSF Protein [NBP2-34884]](https://resources.bio-techne.com/images/products/Human-G-CSF-Protein-SDS-Page-NBP2-34884-img0001.jpg "SDS-PAGE: Recombinant Human G-CSF Protein [NBP2-34884]")
SDS-PAGE: Recombinant Human G-CSF Protein [NBP2-34884]
SDS-Page: Human G-CSF Protein [NBP2-34884]Formulation, Preparation and Storage
NBP2-34884
| Formulation | Lyophilized from a 0.2 um filtered concentrated solution in 10mM sodium acetate buffer, pH 4.0. |
| Preservative | 5% Trehalose |
| Concentration | Lyoph |
| Reconstitution | Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL. Apportion stock solutions into working aliquots and store at |
| Format | Carrier-Free |
| Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles. |
Background: G-CSF
Long Name
Granulocyte Colony Stimulating Factor
Alternate Names
C17orf33, CSF3, CSF3OS, Filgrastim, GCSF, Lenograstim, Pluripoietin
Gene Symbol
CSF3
UniProt
Additional G-CSF Products
Product Documents for Recombinant Human G-CSF Protein
Product Specific Notices for Recombinant Human G-CSF Protein
This lyophilized preparation is stable for 12 months from date of receipt at -20 to -70 degrees C, preferably desiccated. Upon reconstitution, the preparation can be stored for 1 month at 2-8 degrees C under sterile conditions, and for 3 months at -20 degrees C to -70 degrees C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Stock solutions should be aliquoted and stored at < -20 degrees C. Further dilutions should be made in appropriate buffered solutions
This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.
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